Nanocrystalline diamond:: In vitro biocompatibility assessment by MG63 and human bone marrow cells cultures
Nanocrystalline diamond:: In vitro biocompatibility assessment by MG63 and human bone marrow cells cultures
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DOI：
10.1002/jbm.a.31742
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发表时间：
2008-10-01
影响因子：
4.9
通讯作者：
Fernandes, M. H.
中科院分区：
文献类型：
--
作者：
Amaral, M.;Dias, A. G.;Fernandes, M. H.
纳米金刚石（NCD）在假体涂层方面具有很大的应用潜力。然而，它的生物相容性仍然需要更好地理解。为此，我们采用了几种材料表征技术（SEM，AFM，显微拉曼光谱）和使用MG 63成骨细胞样细胞和人骨髓细胞的细胞培养试验。进行了由SEM和共聚焦显微镜表征支持的生化常规（MTT测定、Lowry法、ALP活性）。我们使用氮化硅（Si3N4）基板的NCD涂层的基础上，这些NCD涂层陶瓷的上级附着力和摩擦学性能的先前演示。结果表明，与标准聚苯乙烯组织培养板相比，人成骨细胞增殖和分化标志物（如ALP活性和基质矿化）的刺激得到改善。NCD的纳米特征与其化学亲和力相关，是骨再生目的的关键点。(C)2007 Wiley Periodicals，Inc.
Nanocrystalline diamond (NCD) has a great potential for prosthetic implants coating. Nevertheless, its biocompatibility still has to be better understood. To do so, we employed several materials characterization techniques (SEM, AFM, micro-Raman spectroscopy) and cell Culture assays using MG63 osteoblast-like and human bone marrow, cells. Biochemical routines (MTT assays, Lowry's method, ALP activity) supported by SEM and confocal microscopy characterization were carried out. We used silicon nitride (Si3N4) Substrates for NCD coatings based on a previous demonstration Of the Superior adhesion and tribological performance of these NCD coated ceramics. Results demonstrate an improved human osteoblast proliferation and the stimulation of differentiated markers, like ALP activity and matrix mineralization, compared with standard polystyrene tissue Culture plates. The nanometric featuring of NCD, associated to its chemical affinity are key points for bone regeneration purposes. (C) 2007 Wiley Periodicals, Inc.