Fusion as a mediator of cytolysis in mixtures of uninfected CD4+ lymphocytes and cells infected by human immunodeficiency virus.
Fusion as a mediator of cytolysis in mixtures of uninfected CD4+ lymphocytes and cells infected by human immunodeficiency virus.
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融合作为未感染 CD4 淋巴细胞和人类免疫缺陷病毒感染细胞混合物中细胞溶解的介质。
DOI：
10.1073/pnas.84.5.1429
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发表时间：
1987
影响因子：
11.1
通讯作者：
Hollinger,FB
中科院分区：
文献类型：
--
作者：
Yoffe,B;Lewis,DE;Petrie,BL;Noonan,CA;Melnick,JL;Hollinger,FB
我们描述了一种不寻常的细胞病理学类型，在这种类型中，未感染的CD4+(辅助/诱导)细胞(表达人类白细胞抗原CD4的细胞)与持续感染人类免疫缺陷病毒(HIV)的细胞相互作用。不需要事先的抗原刺激，因为从没有抗HIV抗体的健康人或从有抗HIV抗体的人身上获得的CD4+细胞能够诱导细胞溶解。CD8+细胞(抑制性/细胞毒性)和CD16+细胞(自然杀伤细胞)都不参与这一反应。光镜和放射自显影研究表明，在细胞溶解之前，HIV感染细胞与大量未感染的CD4+淋巴细胞融合形成多核巨细胞。这些数据可能解释了体内存在的悖论，即在存在少量感染艾滋病毒的CD4+细胞的情况下，CD4+淋巴细胞发生戏剧性的耗尽。这些对获得性免疫缺陷综合征(AIDS)发病机制的新见解可能会导致未来的治疗策略。
We describe an unusual type of cytopathology in which uninfected CD4+ (helper/inducer) cells (cells expressing the human leukocyte antigen CD4) interact with cells persistently infected with the human immunodeficiency virus (HIV). Prior antigenic stimulation was not required, since CD4+ cells taken either from healthy persons without anti-HIV antibodies or from individuals with anti-HIV antibodies were capable of inducing cytolysis. Neither CD8+ (suppressor/cytotoxic) nor CD16+ (natural killer) cells mediated the reaction. Light microscopic and autoradiographic studies revealed that, prior to cytolysis, multinucleated giant cells were formed from fusions between HIV-infected cells and large numbers of uninfected CD4+ lymphocytes. These data may explain the paradox that exists in vivo in which a dramatic depletion of CD4+ lymphocytes occurs in the presence of a small number of HIV-infected CD4+ cells. These new insights into the pathogenesis of acquired immunodeficiency syndrome (AIDS) may lead to future therapeutic strategies.