Distinct daf-16 isoforms regulate specification of vulval precursor cells in Caenorhabditis elegans.
Distinct daf-16 isoforms regulate specification of vulval precursor cells in Caenorhabditis elegans.
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DOI：
10.17912/micropub.biology.000706
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发表时间：
2022
影响因子：
--
通讯作者：
Karp, Xantha
中科院分区：
文献类型：
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作者：
Cuko, Liberta;Cale, Allison R;Rambo, Loni;Knoblock, Macy L;Karp, Xantha
FOXO转录因子调节跨物种的发育、寿命和抗逆性。梭线虫FOXO直系同源物daf-16具有三种主要的同种型，其具有不同的启动子和N-末端。同种型的不同组合调节不同的过程。幼虫二次蜕皮后，不利的环境可引起子代滞育。daf-16阻断了外阴前体细胞的特化，包括EGFR/Ras介导的1 β细胞特化和LIN-12/Notch介导的2 β细胞特化。使用同种型特异性突变体，我们发现daf-16 a和daf-16 f对于阻断1-硫代磷酸酯标记物的表达是功能冗余的。相比之下，所有三种同种型都有助于阻断2种脂酶标志物的表达。
FOXO transcription factors regulate development, longevity, and stress-resistance across species. The C. elegans FOXO ortholog, daf-16, has three major isoforms with distinct promoters and N-termini. Different combinations of isoforms regulate different processes. Adverse environments can induce dauer diapause after the second larval molt. During dauer, daf-16 blocks specification of vulval precursor cells, including EGFR/Ras-mediated 1˚ fate specification and LIN-12/Notch-mediated 2˚ fate specification. Using isoform-specific mutants, we find that daf-16a and daf-16f are functionally redundant for the block to the expression of 1˚ fate markers. In contrast, all three isoforms contribute to blocking the expression of 2˚ fate markers.