Plasticity in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits in the rat dorsal horn following deafferentation
Plasticity in α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor subunits in the rat dorsal horn following deafferentation
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DOI：
10.1016/s0304-3940(98)00036-6
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发表时间：
1998-02-06
影响因子：
2.5
通讯作者：
Coggeshall, RE
中科院分区：
文献类型：
--
作者：
Carlton, SM;Hargett, GL;Coggeshall, RE
α -氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体的GluR1和GluR2/3亚基免疫染色在正常背角的I和II层中突出，在更深的层中染色较少。背根切断术三天后，图像分析显示两种抗体在浅背角的染色密度显著降低。相比之下，GluR1仅在III层、IV层和V层网状区域的染色密度显著增加。这种增加表明AMPA受体通过至少两种机制促进神经递质脱神经或神经根性疼痛：(1)AMPA受体深层膜GluR1亚基上调，(2)背角神经元Ca2+通透性增强，因为缺乏GluR2亚基的AMPA受体是Ca2+通透性的。谷氨酸激活这些受体可以启动或增强第二信使级联反应，这可能有助于脱神经动物背角记录的神经元变化。(C) 1998爱思唯尔科学爱尔兰有限公司
Immunostaining for GluR1 and GluR2/3 subunits of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor is prominent in laminae I and II of the normal dorsal horn, with much less staining in deeper laminae. Three days after dorsal rhizotomy, image analysis demonstrates a significant decrease in staining density for both antibodies in the superficial dorsal horn. By contrast, staining density is significantly increased in laminae III, IV and the reticulated region of lamina V for GluR1 only. This increase suggests that AMPA receptors contribute to deafferentation or radicular pain by at least two mechanisms: (1) up-regulation of GluR1 subunits of the AMPA receptor in deeper laminae, and (2) enhanced Ca2+ permeability of dorsal horn neurons because AMPA receptors lacking the GluR2 subunit are Ca2+ permeable. Glutamate activation of these receptors could initiate or potentiate second messenger cascades that could well contribute to neuronal changes documented in the dorsal horn of deafferented animals. (C) 1998 Elsevier Science Ireland Ltd.