Comparison of induced sputum with bronchial wash, bronchoalveolar lavage and bronchial biopsies in COPD
Comparison of induced sputum with bronchial wash, bronchoalveolar lavage and bronchial biopsies in COPD
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DOI：
10.1034/j.1399-3003.2000.15a20.x
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发表时间：
2000-01-01
影响因子：
24.3
通讯作者：
Postma, DS
中科院分区：
文献类型：
--
作者：
Rutgers, SR;Timens, W;Postma, DS
在慢性阻塞性肺疾病(COPD)患者中，诱导痰中的细胞和可溶性炎性标志物与灌洗液和活检组织中的标志物之间的关系尚不清楚，我们对此进行了研究，并分析了COPD患者和健康对照组之间的可能差异。吸入高渗盐水诱导痰。慢性阻塞性肺疾病患者痰中以中性粒细胞为主(中位数为77.3%)，而中性粒细胞在BW(5.5%)和BAL液(1.7%)中没有。痰中细胞分类与BW、BAL液或活检组织中的细胞计数无关，而与BW、BAL液或活检组织中的细胞计数无关，而与BW液、BAL液或活检组织中嗜酸性粒细胞阳离子蛋白水平(RHO=0.66，P=0.007)、痰中白细胞介素8(IL-8)浓度、Vith、BAL和BAL液中的细胞计数呈正相关液体IL-8浓度(Rho=0.52p=0.026)，慢性阻塞性肺疾病患者痰中中性粒细胞和嗜酸性粒细胞百分比高于健康对照组，细胞外钙蛋白和IL-8浓度高于健康对照组。BW和BAL液中的嗜酸性粒细胞百分比和ECP浓度也较高。最后，在活检组织中发现了更多的巨噬细胞和嗜酸性粒细胞。总之，诱导痰来自于BW和BAL液和活检组织的不同隔室。诱导痰可用于研究腔内中性粒细胞和嗜酸性粒细胞在慢性阻塞性肺疾病中的作用。
It is unclear how cellular and soluble inflammatory markers in induced sputum relate to markers in lavage fluid and biopsies in chronic obstructive pulmonary disease (COPD), This was investigated and also the possible differences between subjects with COPD and healthy controls assessed.Eighteen nonatopic subjects with COPD and 11 healthy controls were studied. Sputum was induced by inhalation of hypertonic saline. The airways were lavaged, using the first 50 mt for bronchial wash (BW) and the subsequent 150 mt for bronchoalveolar lavage (BAL), and biopsies were taken from subsegmental carinae.Neutrophils were the predominant cell type in sputum in COPD (median 77.3%) but not in BW (5.5%) and BAL fluid (1.7%), Differential cell counts in sputum did not correlate with the counts in BW or BAL fluid or biopsies, whereas sputum eosinophil cationic protein (ECP) levels correlated with BW fluid ECP levels (rho=0.66, p=0.007) and sputum interleukin-8 (IL-8) concentration,vith BAL, fluid LL-8 concentration (rho= 0.52, p=0.026), Subjects with COPD had a higher percentage of sputum neutrophils and eosinophils and higher concentrations of ECP and IL-8 than healthy controls. The higher percentages of eosinophils and concentrations of ECP were also seen in BW and BAL fluid. Finally, higher numbers of macrophages and eosinophils were found in biopsies.In conclusion, induced sputum is derived from a different compartment from BW and BAL fluid and biopsies. Induced sputum may be useful for studying the contribution of luminal neutrophils and eosinophils in chronic obstructive pulmonary disease.