Manganese-enhanced magnetic resonance imaging of mouse brain after systemic administration of MnCl2:: Dose-dependent and temporal evolution of T1 contrast
Manganese-enhanced magnetic resonance imaging of mouse brain after systemic administration of MnCl2:: Dose-dependent and temporal evolution of T1 contrast
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DOI：
10.1002/mrm.20368
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发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Koretsky, AP
中科院分区：
文献类型：
--
作者：
Lee, JH;Silva, AC;Koretsky, AP
锰是动物磁共振成像的有用造影剂。此前，已有研究表明，全身剂量的MnCl2在啮齿类动物的大脑中提供了独特的对比，使神经结构得以可视化。目前的工作是研究静脉注射后脑增强的剂量和时间依赖性。给小鼠注射不同剂量的MnCl2(9~175 mg/kg)后0~24 h行T_1加权锰增强磁共振成像(MEMRI)，测量不同脑区的MnCl2前T_(-1)值在1.17+/-0.03~1.76+/-0.01 S之间，给药后24小时测得的T_(-1)值显著降低。降幅最大的是脑垂体，最低剂量后为231+/-23ms，最大剂量后为143+/-43ms，皮质最小(低剂量组为1060+/-5ms，高剂量组为637+/-5ms)。14小时后产生的对比度在24小时内没有变化。增强首先发生在蛛网膜下腔，然后是脑室和脑室周围组织，最后到达大脑的其余部分。皮质层在较高剂量(>；88 mg/kg)下检测到，嗅球层在最低剂量(9 mg/kg)下检测到。观察到嗅球各层增强的时间演变。在大脑的一些区域，如海马体和丘脑，在2到14小时之间检测到的对比变化使用了非常特定的路径。这些结果表明，可以控制MnCl2的剂量和时间，以特定区域的方式优化大脑对比度。2005年出版，Wiley-Liss，Inc.
Manganese is a useful contrast agent for MRI of animals. Previously, it has been shown that systemic doses of MnCl2, provide unique contrast in the rodent brain, enabling visualization of neuroarchitecture. The present work investigates the dose and temporal dependence of brain enhancement after i.v. administration of MnCl2 Varying doses of MnCl2 (9-175 mg/kg) were administered to mice from 0 to 24 h prior to T-1 -weighted manganese-enhanced MRI (MEMRI) at 11.7 T. Pre-MnCl2 T-1 values measured in different brain regions ranged from 1.17 +/- 0.03 to 1.76 +/- 0.01 s. Post-MnCl2 T-1 measured 24 hr after administration of MnCl2 were significantly decreased, even after the lowest dose of MnCl2. The largest decreases occurred in the pituitary gland, where post-MnCl2 T-1 ranged from 231 +/- 23 ms following the lowest dose to 143 +/- 43 ms after the highest dose, while the smallest decreases were observed in cortex (post-MnCl2 T-1 = 1060 +/- 5 ms for low dose and 637 +/- 5 ms for high dose). The contrast resulting after 14 hr did not change up to 24 hr. Enhancement first occurred in subarachnoid spaces, followed by ventricles and periventricular tissues, and finally reached the remainder of the brain. Cortical layers were detected at higher doses (>88 mg/kg) and olfactory bulb layers were detected with the lowest dose (9 mg/kg). Temporal evolution of the enhancement of the olfactory bulb layers was observed. In some regions of the brain, such as hippocampus and thalamus, the changes in contrast detected between 2 and 14 hr used very specific pathways. These results demonstrate that both the dose and the time after MnCl2 can be manipulated to optimize brain contrast in a region-specific manner. Published 2005 Wiley-Liss, Inc.