Effect of tumor necrosis factor administration in vivo on lipoprotein lipase activity in various tissues of the rat.
Effect of tumor necrosis factor administration in vivo on lipoprotein lipase activity in various tissues of the rat.
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发表时间：
1989-04
影响因子：
6.5
通讯作者：
Carl Grunfeld;R. Gulli;A. Moser;L. Gavin;K. Feingold
中科院分区：
文献类型：
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作者：
Carl Grunfeld;R. Gulli;A. Moser;L. Gavin;K. Feingold
当添加到培养的小鼠脂肪细胞中时，肿瘤坏死因子 (TNF) 会降低脂蛋白脂肪酶 (LPL) 的水平。 Semb等人(1987.J.Biol Chem.262：8390-8394)已经表明，对大鼠施用鼠TNF可降低附睾脂肪垫中的脂蛋白脂肪酶(LPL)，最大抑制需要数小时。我们现在已经测试了用 TNF 治疗大鼠对多种组织中 LPL 活性的影响，发现在血清甘油三酯急剧升高的条件下，很少有 LPL 出现下降。用人TNF（25微克/200克，静脉注射）治疗雄性大鼠90分钟后，血清甘油三酯升高2.2倍，但附睾脂肪中的LPL活性没有降低。 TNF 治疗 16 小时后，附睾脂肪中的 LPL 活性降低了 44%，与之前报道的数据一致。相比之下，在雌性大鼠中，尽管血清甘油三酯增加（分别为1.8倍和1.5倍），但在施用TNF后90分钟或16小时，宫旁脂肪组织中的LPL活性没有显着降低。 TNF 治疗后，雄性或雌性大鼠的大多数其他脂肪组织部位的 LPL 活性几乎没有变化。在任何时候都没有观察到 TNF 对心脏或膈肌 LPL 的影响。在血清甘油三酯升高的条件下，雄性和雌性大鼠的 TNF 治疗均会导致体内从头肝脂肪生成持续增加。 TNF 对体内 LPL 的有限影响不太可能解释血清甘油三酯的快速和持续增加。
When added to murine adipocytes in culture, tumor necrosis factor (TNF) decreases the levels of lipoprotein lipase (LPL). Semb et al (1987. J. Biol Chem. 262: 8390-8394) have shown that administration of murine TNF to rats decreases lipoprotein lipase (LPL) in the epididymal fat pad with maximal inhibition requiring several hours. We have now tested the effects of treatment of rats with TNF on LPL activity in a variety of tissues and find that few show decreases in LPL under conditions that acutely increase serum triglycerides. Ninety minutes after treatment of male rats with human TNF (25 micrograms/200 g, i.v.), serum triglycerides rose 2.2-fold but there was no decrease in LPL activity in epididymal fat. Sixteen hours after TNF treatment LPL activity had decreased by 44% in epididymal fat, consistent with the previously reported data. In contrast, in female rats, no significant decrease was seen in LPL activity in parametrial adipose tissue at either 90 min or 16 hr after TNF administration despite increases in serum triglycerides (1.8-fold and 1.5-fold, respectively). There was little change in LPL activity in most other adipose tissue sites of male or female rats at either time after TNF treatment. No effect of TNF was seen on heart or diaphragm muscle LPL at any time. TNF treatment of both male and female rats produces consistent increases in de novo hepatic lipogenesis in vivo under conditions that increase serum triglycerides. It is unlikely that the limited effects of TNF on LPL in vivo can account for the rapid and sustained increase in serum triglycerides.