Analysis of chromogranin/secretogranin messenger RNAs in human pituitary adenomas.
Analysis of chromogranin/secretogranin messenger RNAs in human pituitary adenomas.
复制标题
人垂体腺瘤中嗜铬粒蛋白/分泌粒蛋白信使 RNA 的分析。
DOI：
10.1097/00019606-199403010-00007
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Jin,L
中科院分区：
文献类型：
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作者：
Lloyd,RV;Jin,L
：通过原位杂交 (ISH) 分析了 25 个垂体腺瘤的各种嗜铬粒蛋白/分泌粒蛋白 (Cg/Sg) 信使 RNA (mRNA) 转录物的表达。还通过 Northern 杂交分析了另外五个腺瘤。还对 CgA 和 SgIV（使用单克隆抗体 HISL-19）进行了免疫组织化学染色。大多数催乳素和促肾上腺皮质激素腺瘤不表达 CgA mRNA 或蛋白，而生长激素 (GH) 肿瘤通过 Northern 和原位杂交分析具有低至中等量的 CgA mRNA，并且 CgA 蛋白局部呈阳性。大多数腺瘤中存在 CgB、SgII、SgIII 和 SgV mRNA 转录本，并且在所有肿瘤组中均检测到 SgIV 蛋白。培养7天的GH和空细胞腺瘤也表达CgA/Sg mRNA转录物和蛋白质。通过原位杂交分析，一些 CgA 蛋白阴性的腺瘤石蜡切片可检测到 CgA mRNA。这些结果表明，与其他类型的垂体腺瘤相比，CgA mRNA 和蛋白质在产生糖蛋白激素的肿瘤中更常见，并且即使免疫组织化学未检测到 CgA 蛋白，CgA 的 ISH 也可以检测到 CgA 的 mRNA 转录本。
: Twenty-five pituitary adenomas were analyzed for expression of various chromogranin/secretogranin (Cg/Sg) messenger RNA (mRNA) transcripts by in situ hybridization (ISH). An additional five adenomas were also analyzed by Northern hybridization. Immunohistochemical staining for CgA and for SgIV (with monoclonal antibody HISL-19) was also performed. Most prolactin and adrenocorticotropin adenomas did not express CgA mRNA or protein, whereas growth hormone (GH) tumors had low to moderate amounts of CgA mRNA by Northern and in situ hybridization analyses and were focally positive for CgA protein. CgB, SgII, SgIII, and SgV mRNA transcripts were present in most adenomas, and SgIV protein was detected in all groups of tumors. A GH and a null cell adenoma cultured for 7 days also expressed CgA/Sg mRNA transcripts and protein. Paraffin sections of some adenomas that were negative for CgA protein had detectable CgA mRNA by in situ hybridization analysis. These results indicate that CgA mRNA and protein are more commonly expressed in glycoprotein hormoneproducing tumors compared with other types of pituitary adenomas and that ISH for CgA may detect the mRNA transcripts for CgA even when CgA protein is not detected by immunohistochemistry.