B cell subpopulations separated by CD27 and crucial collaboration of CD27(+) B cells and helper T cells in immunoglobulin production
B cell subpopulations separated by CD27 and crucial collaboration of CD27(+) B cells and helper T cells in immunoglobulin production
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DOI：
10.1002/eji.1830270835
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发表时间：
1997-08-01
影响因子：
5.4
通讯作者：
Komiyama, A
中科院分区：
文献类型：
--
作者：
Agematsu, K;Nagumo, H;Komiyama, A
B细胞免疫球蛋白的产生通过直接相互作用和分泌的细胞因子来调节辅助T细胞。在本研究中，我们在脐带和外周血B细胞中功能分析了CD27。成年外周血B细胞分为CD27（+）和CD27（ - ）细胞，其形态不同。脐带血B细胞未表达CD27，周围血液B细胞上的CD27表达随着年龄的增长而增加。只有CD27（+）B细胞具有产生免疫球蛋白的能力，通过与肿瘤坏死因子相关的跨膜配体CD70的接触增加。成人外周血CD27（+）B细胞可以进一步细分为两个离散亚型：IgD（ - ）CD27（+）和IgD（+）CD27（+）B细胞。 IgD（ - ）CD27（+）B细胞产生IgG，IgM和IgA，而IgD（+）CD27（+）B细胞主要产生IgM。表达CD70表达CD70的活化CD4（+）CD45RO T细胞在活化的B细胞上表达CD27表达下调，并且这种下调被抗CD70单克隆抗体完全阻断，表明通过CD27/CD70直接T-B细胞接触。通过CD27和CD40触发的触发性可添加性地增加了金黄色葡萄球菌Cowan菌株和白介素2刺激下的免疫球蛋白产生。综上所述，我们的发现表明，通过CD27和IGD表达将外周血B细胞分离为亚群，并且CD27（+）B细胞通过与CD70分子相互作用而产生大量免疫球蛋白。
B cell immunoglobulin production is regulated by helper T cells through direct interaction and secreted cytokines. In the present study, we functionally analyzed CD27 in cord and peripheral blood B cells. Adult peripheral blood B cells were separated into CD27(+) and CD27(-) cells, which differed in their morphology. Cord blood B cells did not express CD27, and CD27 expression on peripheral blood B cells increased with age. Only CD27(+) B cells had the ability to produce immunoglobulin, which was increased by contact with a tumor necrosis factor-related transmembrane ligand, CD70. Adult peripheral blood CD27(+) B cells can be further subdivided into two discrete subtypes: IgD(-) CD27(+) and IgD(+) CD27(+) B cells. IgD(-) CD27(+) B cells produce IgG, IgM and IgA, whereas IgD(+) CD27(+) B cells predominantly produce IgM. The addition of activated CD4(+)CD45RO T cells expressing CD70 caused down-regulation of CD27 expression on activated B cells, and this down-modulation was completely blocked by anti-CD70 monoclonal antibody, indicating direct T-B cell contact via CD27/CD70. The triggering via CD27 and CD40 additively increased the immunoglobulin production under Staphylococcus aureus Cowan strain plus interleukin-2 stimulation. Taken together, our findings demonstrate that peripheral blood B cells are separated into subpopulations by CD27 and IgD expression and that CD27(+) B cells produce large amounts of immunoglobulin by interaction with the CD70 molecule.