Hypermethylation and prognostic implication of Syk gene in human colorectal cancer
Hypermethylation and prognostic implication of Syk gene in human colorectal cancer
复制标题
Syk 基因在人类结直肠癌中的高甲基化及其预后意义
DOI：
10.1007/s12032-013-0586-8
复制
发表时间：
2013-04
期刊：
影响因子：
3.4
通讯作者：
Yuan, Yunfei
中科院分区：
文献类型：
--
作者：
Wang, Jianping;Huo, Lijun;Chen, Hao;Ni, Beibei;Xiang, Jun;Kang, Liang;Wang, Lei;Peng, Junsheng;Yuan, Yunfei
本研究旨在探讨Syk基因甲基化与结直肠癌临床病理特征及远期预后的关系。还评估了Syk对细胞增殖和侵袭能力的影响。采用MSP、qRT-PCR和western blot检测结直肠癌组织和细胞系中Syk的甲基化和表达状态。通过体外实验研究Syk过表达对肿瘤发生的影响。还分析了结直肠癌患者中Syk甲基化与临床相关性之间的相关性。Syk甲基化在CRC组织样品中发现48.6%，在细胞系中分别发现57.1%。去甲基化剂可使Syk表达恢复。Syk在大肠癌细胞中的过表达抑制了大肠癌细胞的增殖（p< 0.01）和侵袭（p< 0.01）。Syk基因甲基化与组织学分级（p= 0.002）、淋巴结状态（p< 0.001）和TNM分期（p< 0.001）显著相关。甲基化Syk组的5年总生存率显著低于非甲基化Syk组（59 vs. 80%，p< 0.001）。多因素分析表明Syk甲基化是影响总生存率的独立预后因素。Syk在CRC进展中被鉴定为潜在的肿瘤抑制因子。Syk甲基化与总生存率相关，可作为CRC的独立预后指标。
The study was aimed to investigate the relationship between hypermethylation of Syk gene and clinicopathological characteristics and long-term outcomes in colorectal cancer. The effect of Syk on cell proliferation and invasion ability was also assessed. Methylation and expression status of Syk were explored in CRC tissues and cell lines by MSP, qRT-PCR and western blot assay. The effects of Syk overexpression on tumorigenesis were studied by in vitro assay. The correlation between Syk methylation and clinical relevance in CRC patients was also analyzed. Syk methylation was found 48.6 % in CRC tissue samples and 57.1 % in cell lines, respectively. The loss of Syk expression could be restored by demethylation agent. Overexpression of Syk in CRC cell inhibited cell proliferation (p< 0.01) and invasion (p< 0.01). The methylation of Syk was significantly associated with histological grade (p= 0.002), lymph node status (p< 0.001) and TNM stage (p< 0.001). Five-year overall survival in methylated Syk group was significantly lower than that in unmethylated Syk group (59 vs. 80 %,p< 0.001). Multivariate analysis demonstrated that Syk methylation was an independent prognostic factor for overall survival. Syk is identified as a potential tumor suppressor in CRC progression. Syk methylation is correlated with poor overall survival, which acts as an independent prognostic indicator of CRC.
登录
查看更多内容
影响因子：
11.2
作者：
Yunfei Yuan;Richard Mendez;Aysegul A. Sahin;J. Dai
通讯作者：
Yunfei Yuan;Richard Mendez;Aysegul A. Sahin;J. Dai
DOI：
10.1200/jco.1998.16.2.793
发表时间：
1998-02
期刊：
Journal of clinical oncology : official journal of the American Society of Clinical Oncology
影响因子：
--
作者：
R. Bast;C. Desch;D. Hayes;N. Kemeny;P. Ravdin;M. Somerfield;Thomas J. Smith
通讯作者：
R. Bast;C. Desch;D. Hayes;N. Kemeny;P. Ravdin;M. Somerfield;Thomas J. Smith
影响因子：
6.4
作者：
Ogane, Satoru;Oncla, Takeshi;Shibahara, Takahiko
通讯作者：
Shibahara, Takahiko
影响因子：
2.7
作者：
Tsuchida, S;Yanagi, S;Yamamura, H
通讯作者：
Yamamura, H
DOI：
10.1186/bcr261
发表时间：
2001
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Stewart ZA;Pietenpol JA
通讯作者：
Pietenpol JA