Yeast Cls2p/Csg2p localized on the endoplasmic reticulum membrane regulates a non‐exchangeable intracellular Ca2+ pool cooperatively with calcineurin
Yeast Cls2p/Csg2p localized on the endoplasmic reticulum membrane regulates a non‐exchangeable intracellular Ca2+ pool cooperatively with calcineurin
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位于内质网膜上的酵母 Cls2p/Csg2p 与钙调神经磷酸酶协同调节不可交换的细胞内 Ca2+ 池
DOI：
10.1016/0014-5793(95)01478-0
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发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
Y. Anraku
中科院分区：
文献类型：
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作者：
I. Tanida;Yoko Takita;Akira Hasegawa;Y. Ohya;Y. Anraku
酿酒酵母CLS 2基因产物（Cls2p）定位于内质网，对胞内不同于液泡的一个区室中的Ca 2+的调节起重要作用。使用avma3突变，削弱了钙离子进入vocuole的螯合活性，我们已经表明，cls2突变导致不能与细胞外钙离子交换的钙离子池增加3.4倍。加入免疫抑制剂FK506可协同升高Thecls2细胞内Ca 2+的蓄积。此外，在vma 3背景下，FK506大大增强了cls 2突变引起的毒性。鉴于FK506抑制钙调磷酸酶活性，Cls2p可能在从内质网释放Ca2+通量中起作用，以某种方式与钙调磷酸酶合作。
Saccharromyces cerevisiae CLS2gene product (Cls2p) that is localized on the endoplasmic reticulum is important for the regulation of intracellular Ca2+in a compartment distinct from the vacuole. Using avma3mutation that impairs the Ca2+sequestering activity into the vocuole, we have shown that thecls2mutation results in 3.4‐fold increase in the Ca2+pool that is not exchangeable with extracellular Ca2+. Accumulation of Ca2+within thecls2cells is synergistically elevated by the addition of immunosuppressant, FK506. Moreover, in thevma3background, toxicity caused by thecls2mutation is greatly enhanced by FK506. Given that FK506 inhibits the calcineurin activity, Cls2p likely functions in releasing Ca2+flux from the endoplasmic reticulum, somehow cooperating with calcineurin.
DOI：
10.1042/bj20131429
发表时间：
2014-03-15
期刊：
The Biochemical journal
影响因子：
--
作者：
Mustafi SM;Brecher M;Zhang J;Li H;Lemaster DM;Hernández G
通讯作者：
Hernández G