Genetic Diversity and Characterization of a Core Collection of Malus Germplasm Using Simple Sequence Repeats (SSRs)

Genetic Diversity and Characterization of a Core Collection of Malus Germplasm Using Simple Sequence Repeats (SSRs)
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DOI:
10.1007/s11105-011-0399-x
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发表时间:
2012-08-01
影响因子:
2.1
通讯作者:
Korban, Schuyler S.
Korban, Schuyler S.
中科院分区:
生物学4区
文献类型:
--
作者:
Potts, Sarah M.;Han, Yuepeng;Korban, Schuyler S.

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利用简单重复序列(SSR)技术对苹果属植物种质资源的遗传多样性和亲缘关系进行了研究。共有164个加入苹果核心收集,保持在伊利诺伊大学,基因型使用苹果SSR标记。每个加入的基因型使用一个单一的强大的SSR标记,从每一个17个不同的连锁群在苹果。数据进行主成分分析,并构建树状图,以建立遗传相关性。正如预期的那样,这个多样化的核心集合显示出较高的等位基因多样性,而且,这种等位基因多样性高于以前报道的。聚类分析表明,在这个集合中加入四个不同的集群的存在。
Simple sequence repeats (SSRs) were used to assess genetic diversity and study genetic relatedness in a large collection of Malus germplasm. A total of 164 accessions from the Malus core collection, maintained at the University of Illinois, were genotyped using apple SSR markers. Each of the accessions was genotyped using a single robust SSR marker from each of the 17 different linkage groups in Malus. Data were subjected to principal component analysis, and a dendrogram was constructed to establish genetic relatedness. As expected, this diverse core collection showed high allelic diversity; moreover, this allelic diversity was higher than that previously reported. Cluster analysis revealed the presence of four distinct clusters of accessions in this collection.