The danger of metabolic pathways with turbo design
The danger of metabolic pathways with turbo design
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DOI：
10.1016/s0968-0004(98)01205-5
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发表时间：
1998-05-01
影响因子：
13.8
通讯作者：
Westerhoff, HV
中科院分区：
文献类型：
--
作者：
Teusink, B;Walsh, MC;Westerhoff, HV
许多分解代谢途径开始需要ATP的激活步骤，之后进一步代谢产生过剩的ATP。这种“涡轮”原理很有用，但也包含固有的风险。这是说明了一个矛盾的酿酒酵母突变体的详细动力学分析，突变体不能生长在葡萄糖上，因为过度活跃的糖酵解的初始酶，但只有在一个酶（海藻糖6-磷酸合酶），似乎没有什么相关的糖酵解缺陷。具有初始激活步骤的途径的普遍存在表明，可能有更多的基因，当删除时，会导致相当矛盾的调节表型（即生长底物利用增强引起的生长缺陷）。
Many catabolic pathways begin with an ATP-requiring activation step, after which further metabolism yields a surplus of ATP. Such a 'turbo' principle is useful but also contains an inherent risk. This is illustrated by a detailed kinetic analysis of a paradoxical Saccharomyces cerevisiae mutant; the mutant fails to grow on glucose because of overactive initial enzymes of glycolysis, but is defective only in an enzyme (trehalose 6-phosphate synthase) that appears to have little relevance to glycolysis. The ubiquity of pathways that possess an initial activation step, suggests that there might be many more genes that, when deleted, cause rather paradoxical regulation phenotypes (i.e. growth defects caused by enhanced utilization of growth substrate).