A technique to prevent dural adhesions to chronically implanted microelectrode arrays
A technique to prevent dural adhesions to chronically implanted microelectrode arrays
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DOI：
10.1016/s0165-0270(00)00159-x
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发表时间：
2000-04-15
影响因子：
3
通讯作者：
Normann, RA
中科院分区：
文献类型：
--
作者：
Maynard, EM;Fernandez, E;Normann, RA
最大限度地减少神经组织和长期植入其中的微电极阵列之间的相对运动有望大大增强微电极长期记录单个皮层神经元的能力。我们描述了一种新的手术技术，使用位于阵列和硬脑膜之间的 12 μm (0.5 mil) 厚的 Teflon(R) 薄膜，最大限度地减少硬脑膜和植入电极阵列之间粘连的形成。共有 15 只猫使用这种技术进行了植入。当Teflon膜保持在其初始位置时，在处死时对12个植入部位进行的肉眼检查未能发现阵列和硬脑膜之间粘连的证据。在进行记录的 6 个植入物中，每个阵列中 11 个连接的电极中平均有 9 个（81%）记录了植入后 180 天后诱发的神经活动。此外，平均而言，在这些阵列中的每个植入电极上识别出两个可分离的单元。四个植入电极阵列的植入电极周围的细胞体密度没有发现显着变化。然而，植入部位的组织学评估揭示了阵列下方脑膜增殖的证据。所描述的技术被证明可以有效地防止植入电极阵列之间的粘连，并改善用这些结构获得的慢性记录的特性。 (C) 2000 Elsevier Science B.V. 保留所有权利。
Minimizing relative movements between neural tissues and arrays of microelectrodes chronically implanted into them is expected to greatly enhance the capacity of the microelectrodes to record from single cortical neurons on a long-term basis. We describe a new surgical technique to minimize the formation of adhesions between the dura and an implanted electrode array using a 12 mu m (0.5 mil) thick sheet of Teflon(R) film positioned between the array and the dura. A total of 15 cats were implanted using this technique. Gross examination of 12 implant sites at the time of sacrifice failed to find evidence of adhesions between the arrays and the dura when the Teflon(R) film remained in its initial position. In six implants from which recordings were made, an average of nine of the 11 (81%) connected electrodes in each array recorded evoked neural activity after 180 days post implantation. Further, on average, two separable units were identified on each of the implanted electrodes in these arrays. No significant change was found in the density of cell bodies around implanted electrodes of four of the implanted electrode arrays. However, histological evaluation of the implant sites revealed evidence of meningeal proliferation beneath the arrays. The technique described is shown to be effective at preventing adhesions between implanted electrode arrays and improve the characteristics of chronic recordings obtained with these structures. (C) 2000 Elsevier Science B.V. All rights reserved.