Redox analysis of human plasma allows separation of pro-oxidant events of aging from decline in antioxidant defenses
Redox analysis of human plasma allows separation of pro-oxidant events of aging from decline in antioxidant defenses
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DOI：
10.1016/s0891-5849(02)01040-7
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发表时间：
2002-11-01
影响因子：
7.4
通讯作者：
Sternberg, P
中科院分区：
文献类型：
--
作者：
Jones, DP;Mody, VC;Sternberg, P
氧化应激是与衰老相关的疾病和退行性过程的组成部分。然而，没有方法可以单独评估与保护性抗氧化系统功能下降有关的致病性氧化事件。先前的研究表明，正在进行的氧化过程使血浆半胱氨酸/胱氨酸氧化还原维持在比抗氧化剂谷胱甘肽/谷胱甘肽二硫化物（GSH/GSSG）系统氧化程度更高的值，这表明这些血浆硫醇的氧化还原分析可以单独评价氧化事件的增加与抗氧化功能的下降。本研究使用122名年龄在19-85岁之间的健康个体血浆中半胱氨酸/胱氨酸和GSH/GSSG氧化还原的测量来确定硫醇-二硫化物氧化还原是否随年龄发生变化。结果表明，半胱氨酸/胱氨酸氧化还原状态的线性氧化与年龄在0.16 mV/年的速率在整个年龄跨度。相反，GSH/GSSG氧化还原在45年之前没有被氧化，随后以0.7 mV/年的几乎线性速率被氧化。这些数据表明，有一个连续的，线性增加的氧化事件在整个成年生活，但谷胱甘肽抗氧化系统的能力，直到45年，然后迅速下降。这些数据进一步表明，半胱氨酸/胱氨酸和GSH/GSSG的氧化还原状态提供了一种方法，以临床区分增加的致病性氧化事件和降低的GSH抗氧化功能。原则上，这种分析可用于评估在衰老和年龄相关疾病的临床症状发作之前或之后早期针对氧化应激的干预策略的功效。(C)2002年爱思唯尔科学公司
Oxidative stress is a component of diseases and degenerative processes associated with aging. However, no means are available to assess causative oxidative events separately from decline in function of protective antioxidant systems. Previous studies show that ongoing oxidative processes maintain plasma cysteine/cystine redox at a value that is more oxidized than the antioxidant glutathione/glutathione disulfide (GSH/GSSG) system, suggesting that redox analysis of these plasma thiols could allow separate evaluation of an increase in oxidative events from a decline in antioxidant function. The present study uses measurement of cysteine/cystine and GSH/GSSG redox in plasma of 122 healthy individuals aged 19-85 years to determine whether thiol-disulfide redox changes occur with age. The results show a linear oxidation of cysteine/cystine redox state with age at a rate of 0.16 mV/year over the entire age span. In contrast, GSH/GSSG redox was not oxidized prior to 45 years and subsequently was oxidized at a nearly linear rate of 0.7 mV/year. These data suggest that there is a continuous, linear increase in oxidative events throughout adult life but that the capacity of the GSH antioxidant system is maintained until 45 years and then declines rapidly. The data further suggest that redox states of cysteine/cystine and GSH/GSSG provide an approach to clinically distinguish between increased causative oxidative events and decreased GSH antioxidant function. In principle, such analyses can be used to assess efficacy of intervention strategies against oxidative stress prior to or early after onset of clinical symptoms in aging and age-related disease. (C) 2002 Elsevier Science Inc.