A simple method to improve the reliability of iontophoretic administration of tracer substances.
A simple method to improve the reliability of iontophoretic administration of tracer substances.
复制标题
一种提高示踪物质离子电渗疗法可靠性的简单方法。
DOI：
10.1016/0165-0270(94)90125-2
复制
发表时间：
1994
影响因子：
3
通讯作者：
Gordon,FJ
中科院分区：
文献类型：
--
作者：
Yu,D;Gordon,FJ
微离子电渗疗法是一种广泛使用的技术，用于将示踪材料沉积到中枢神经系统中用于神经解剖学实验。然而，离子电渗疗法注射的可靠性往往不是最佳的。用硅树脂涂覆玻璃微量移液器的内腔以降低表面张力，将麦胚凝集素-辣根过氧化物酶（WGA-HRP）离子电渗沉积失败的发生率从40%降低到零。用其他示踪物质也观察到类似的结果。对玻璃微量移液器的内表面进行硅化处理可以显著提高离子电渗沉积的可靠性。
Microiontophoresis is a widely used technique for depositing tracer materials into the central nervous system for neuroanatomical experiments. However, the reliability of iontophoretic injection is often less than optimal. Coating the lumen of glass micropipettes with silicone to reduce surface tension reduced the incidence of failure for iontophoretic deposition of wheat germ agglutinin-horseradish peroxidase (WGA-HRP) from 40% to zero. Similar results were observed with other tracer substances. Siliconizing the internal surface of glass micropipettes can significantly improve the reliability of iontophoretic deposition.