Urinary mitochondrial DNA is a useful biomarker for assessing kidney injury of antineutrophil cytoplasmic antibody -associated vasculitis.
Urinary mitochondrial DNA is a useful biomarker for assessing kidney injury of antineutrophil cytoplasmic antibody -associated vasculitis.
复制标题
尿液线粒体 DNA 是评估抗中性粒细胞胞质抗体相关性血管炎肾损伤的有用生物标志物
DOI：
10.1016/j.cca.2019.11.014
复制
发表时间：
2020
影响因子：
5
通讯作者：
Yan TieKun
中科院分区：
文献类型：
--
作者：
Wu SiJing;Yang Xia;Xu PengCheng;Chen Tong;Gao Shan;Hu ShuiYi;Wei Li;Yan TieKun
研究了尿线粒体DNA （mtDNA）在抗中性粒细胞细胞质抗体（ANCA）相关血管炎（AAV）肾损伤评估中的价值。方法对39例肾活检证实髓过氧化物酶(MPO)-ANCA相关的AAV患者进行分析。结果患者尿mtDNA平均值显著高于正常对照组（3372.74±1859.72比474.90±123.59拷贝/nmol肌酐，p < 0.001）。发病时需要透析的患者尿mtDNA水平最高（5072.23±1302.87拷贝/nmol肌酐）。尿mtDNA与尿中性粒细胞明胶酶相关脂钙蛋白呈正相关（R = 0.661, P < 0.001），与肾小球滤过率呈负相关（R = - 0.515, P = 0.001）。新月类尿mtDNA水平（4703.08±1744.31拷贝/nmol肌酐）高于混合类（3258.14±1158.99拷贝/nmol肌酐）和局灶类（2268.15±1897.63拷贝/nmol肌酐）。单因素相关分析显示，尿mtDNA与肾活检中间质中性粒细胞（R = 0.471, P = 0.048）和肾小球中性粒细胞（R = 0.673, P = 0.002）呈正相关。在13例发病时需要血液透析的患者中，10例肾脏恢复患者的尿mtDNA高于3例继续依赖透析的患者（5455.20±1174.64 vs 3795.67±893.34 copy/nmol, p = 0.047）。结论AAV肾损伤患者血清mtDNA升高，其水平与肾损伤严重程度及病理中性粒细胞浸润有关。
BackgroundThe value of urinary mitochondrial DNA (mtDNA) for assessing kidney injury of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) was investigated.MethodsThirty-nine kidney biopsy-proved myeloperoxidase (MPO)-ANCA associated AAV patients were enrolled and analyzed.ResultsThe average urinary mtDNA of patients was significantly higher than that of normal controls (3372.74 ± 1859.72 vs. 474.90 ± 123.59 copy/nmol creatinine, p < 0.001). The patients who needed dialysis at disease onset had the highest levels of urinary mtDNA (5072.23 ± 1302.87 copy/nmol creatinine). Urinary mtDNA positively correlated with urinary neutrophil gelatinase-associated lipocalin (R = 0.661, P < 0.001) and negatively correlated with estimated glomerular filtration rate (R = −0.515, P = 0.001). The urinary mtDNA level of crescentic class (4703.08 ± 1744.31 copy/nmol creatinine) was higher than that of mixed class (3258.14 ± 1158.99 copy/nmol creatinine) and focal class (2268.15 ± 1897.63 copy/nmol creatinine). Univariate correlation analysis showed urinary mtDNA positively correlated with interstitial neutrophils (R = 0.471, P = 0.048) and glomerular neutrophils (R = 0.673, P = 0.002) in kidney biopsy. Among 13 patients who needed hemodialysis at disease onset, 10 patients who got renal recovery had higher urinary mtDNA than 3 patients who remained dialysis dependent (5455.20 ± 1174.64 vs. 3795.67 ± 893.34 copy/nmol creatinine, p = 0.047).ConclusionsUrinary mtDNA increases in AAV with kidney injury, and its levels correlate with the severity of kidney injury and neutrophils infiltration in pathology.