Characterization of the synthesis and secretion of transforming growth factor-alpha from salivary glands and saliva.
Characterization of the synthesis and secretion of transforming growth factor-alpha from salivary glands and saliva.
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DOI：
10.1210/endo.134.2.8299590
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发表时间：
1994-02
期刊：
影响因子：
4.8
通讯作者：
M. G. Humphreys-Beher;S. Macauley;N. Chegini;G. B. V. Setten;K. Purushotham;Carol M. Stewart;Timothy T. Wheeler;Gregory S. Schultz
中科院分区：
文献类型：
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作者：
M. G. Humphreys-Beher;S. Macauley;N. Chegini;G. B. V. Setten;K. Purushotham;Carol M. Stewart;Timothy T. Wheeler;Gregory S. Schultz
通过RIA分析发现，从大鼠、小鼠和人身上收集的全唾液含有高浓度的转化生长因子- α (TGF - α)。TGF - α在未刺激的人唾液（年龄，30-45岁；n = 10; 1.5 +/- 3.1 nM）中的浓度随年龄（年龄，55-70岁；n = 10; 0.4 +/- 0.1 nM）而降低，但在口腔病理（年龄，57-70岁；n = 6; 0.8 +/- 0.2 nM）和Paget病（年龄，58-76岁；n = 8; 2.0 +/- 0.6 nM）中升高。TGF - α在大鼠和小鼠唾液腺中的免疫组织化学定位显示，腮腺和颌下腺的颗粒导管细胞具有特异性免疫染色。对大鼠和小鼠腮腺和颌下腺的总RNA进行逆转录和聚合酶链反应扩增，结果表明TGF - α mRNA存在，提示由唾液腺内源性合成。因此，唾液腺似乎是口腔中第二个表皮生长因子样生长因子家族成员的外分泌来源。
Whole saliva collected from rat, mouse, and human sources was found to contain high concentrations of transforming growth factor-alpha (TGF alpha) when analyzed by RIA. The concentrations of TGF alpha in unstimulated human saliva (age, 30-45 yr; n = 10; 1.5 +/- 3.1 nM) was reduced with age (age, 55-70 yr; n = 10; 0.4 +/- 0.1 nM), but increased in oral pathologies manifested in xerostomia (age, 57-70; n = 6; 0.8 +/- 0.2 nM) and Paget's disease (age, 58-76; n = 8; 2.0 +/- 0.6 nM). Immunohistochemical localization of TGF alpha in the salivary glands of rats and mice revealed specific immunostaining of the granular ductal cells of the parotid and submandibular glands. Reverse transcription followed by polymerase chain reaction amplification of total RNA from the parotid and submandibular glands of rats and mice demonstrated the presence of TGF alpha mRNA, suggesting endogenous synthesis by the salivary glands. Thus, salivary glands appear to be an exocrine source for a second member of the epidermal growth factor-like growth factor family in the oral cavity.