[Complete nucleotide sequence analysis of Bombyx mori Densonucleosis virus type 3 VD2 (China isolate)].

[Complete nucleotide sequence analysis of Bombyx mori Densonucleosis virus type 3 VD2 (China isolate)].
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发表时间：
2006-06
期刊：
Wei sheng wu xue bao = Acta microbiologica Sinica
影响因子：
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通讯作者：
Yong-jie Wang;Keping Chen;Q. Yao;Gui-Tian Gao;X. Han
Yong-jie Wang;Keping Chen;Q. Yao;Gui-Tian Gao;X. Han
中科院分区：
其他
文献类型：
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作者：
Yong-jie Wang;Keping Chen;Q. Yao;Gui-Tian Gao;X. Han

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家蚕致密核分裂病毒3型（中国分离株）基因组包含两种不同的单链线性DNA分子（VD1, VD2）。纯化VD2并将其克隆到pUC119载体上，确定了VD2的全核苷酸序列。序列分析显示，VD2基因组序列全长6022个nts，其中524个nts为倒置末端重复序列（ITRs）。在病毒基因组中，鉴定了正链上的两个主要开放阅读框（ORF1和ORF2）和互补链上的一个次要开放阅读框（ORF3）。计算机分析表明，正链ORF1和负链ORF3最有可能编码主要的非结构蛋白，而正链ORF2最有可能编码主要的结构蛋白。BmDNV-3 VD2与BmDNV-2 VD2 （Yamanashi分离物）的全基因组序列比较表明，它们在VD2区域共有97.7%的基因组序列，在BmDNV-3的VD2区域有132个核苷酸替换，11个核苷酸缺失和2个核苷酸插入。结果表明，BmDNV-3与BmDNV-2亲缘关系密切，但存在一定差异，有助于更好地了解病毒的变异，并为其进化提供线索。
The genome of Bombyx mori densonucleosis virus type 3 (China isolate) contains two kinds different single-stranded linear DNA molecules (VD1, VD2). The VD2 was purified and cloned into the pUC119 vector, and the complete nucleotide sequence of VD2 was determined. Sequence analysis showed that the VD2 genome sequence consisted of 6022 nts, including inverted terminal repeats (ITRs) of 524 nts. In the viral genome, two major open reading frames (ORF1 and ORF2) in the plus strand and one minor ORF (ORF3) in the complementary strand were identified. Computer analysis suggested the plus stand ORF1 and the minus strand ORF3 most likely encode the major nonstructural protein, while the plus stand ORF2 most likely encodes the major structural protein. Comparing the complete genome sequence of BmDNV-3 VD2 with that of BmDNV-2 VD2 (Yamanashi isolate) demonstrated that they shared 97.7% genome sequence in the VD2 region, with substitutions of 132 nucleotides, deletions of 11 nucleotides and insertions of 2 nucleotides in the VD2 region of BmDNV-3. The results suggested that BmDNV-3 is closely related to BmDNV-2, but with some differences, giving a better understanding about the variation of the viruses and providing clues to their evolution.