The direct in vitro effect of insulin-like growth factors (IGFs) on normal bovine mammary cell proliferation and production of IGF binding proteins.
The direct in vitro effect of insulin-like growth factors (IGFs) on normal bovine mammary cell proliferation and production of IGF binding proteins.
复制标题
胰岛素样生长因子 (IGF) 对正常牛乳腺细胞增殖和 IGF 结合蛋白产生的直接体外影响。
作者：
M. McGrath;R. Collier;D. Clemmons;W. Busby;C. Sweeny;G. Krivi
使用胶原蛋白基质在体外培养从妊娠、非泌乳小母牛中分离的乳腺上皮细胞。使用这些系统，胰岛素生长因子-1（IGF-1）的截短形式（des-3-IGF-1）、IGF-1和IGF-2都刺激细胞增殖的显著（0.5至1倍）增加（des-3-IGF-1大于IGF-1大于IGF-2）。当在含有血清和IGF-1的培养基中生长时，正常牛乳腺细胞也产生并分泌至少四种IGF结合蛋白（IGFBP），范围从21 K到48 K（如配体印迹分析所示）。然而，在无血清培养基中生长的细胞仅分泌可检测量的34 K和48 K的2种主要形式的IGFBP。利用免疫印迹分析，这些蛋白被鉴定为IGFBP-2和IGFBP-3，分别。通过向无血清培养基中添加IGF，两种蛋白质都是可诱导的（相对效力; IGF-1大于des-3-IGF-1大于IGF-2）。使用RIA分析，与分泌约1.0ng/ml的对照培养物相比，在IGF-1存在下培养的牛乳腺细胞产生20-25 ng/ml IGFBP-2。暴露于des-3-IGF-1的细胞产生的IGFBP-2减少40-60%，而胰岛素和IGF-2不刺激显著的IGFBP-2产生。这些数据表明，正常牛乳腺细胞分泌IGFBP-2和IGFBP-3。这种分泌受IGF-1和des-3-IGF-1刺激，表明调节局部IGF活性的机制。
Mammary epithelial cells isolated from pregnant, nonlactating heifers were grown in vitro using collagen substrates. Using these systems, the truncated form of insulin growth factor-1 (IGF-1) (des-3-IGF-1), IGF-1, and IGF-2 all stimulated a significant (0.5 to 1 fold) increase in cell proliferation (des-3-IGF-1 greater than IGF-1 greater than IGF-2). When grown in media containing serum plus IGF-1, normal bovine mammary cells also produced and secreted at least four species of IGF-binding protein (IGFBP) ranging from 21K to 48K (as demonstrated by ligand blot analysis). However, cells grown in serum free media secreted detectable quantities of only 2 major forms of IGFBP of 34K and 48K. Using immunoblot analysis, these proteins were identified as IGFBP-2 and IGFBP-3, respectively. Both proteins were inducible by the addition of IGF to the serum free media (relative potency; IGF-1 greater than des-3-IGF-1 greater than IGF-2). Using RIA analysis, bovine mammary cells cultured in the presence of IGF-1 produced 20-25 ng/ml IGFBP-2 compared to control cultures which secrete approximately 1.0 ng/ml. Cells exposed to des-3-IGF-1 produced 40-60% less IGFBP-2 whereas insulin and IGF-2 did not stimulate significant IGFBP-2 production. These data indicate that normal bovine mammary cells secret IGFBP-2 and IGFBP-3. This secretion is stimulated by IGF-1 and des-3-IGF-1 suggesting a mechanism for regulating local IGF activity.
人乳腺癌细胞中的胰岛素样生长因子结合蛋白：与 hIGFBP-2 和 hIGFBP-3 的关系。
DOI：
10.1210/jcem-71-2-530
发表时间：
1990
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
DeLeon,DD;Bakker,B;Wilson,DM;Lamson,G;Rosenfeld,RG
通讯作者：
Rosenfeld,RG
影响因子：
4.8
作者：
CLEMMONS, DR;CAMACHOHUBNER, C;OSBORNE, CK
通讯作者：
OSBORNE, CK
生长调节素-C 替代胰岛素，用于在无血清胶原凝胶细胞培养物中生长正常处女小鼠的乳腺上皮细胞。
影响因子：
4.8
作者：
Imagawa,W;Spencer,EM;Larson,L;Nandi,S
通讯作者：
Nandi,S