Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue
Gender differences in the regulation of P450 aromatase expression and activity in human adipose tissue
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DOI：
10.1038/sj.ijo.0801254
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发表时间：
2000-07-01
影响因子：
4.9
通讯作者：
Kumar, S
中科院分区：
文献类型：
--
作者：
McTernan, PG;Anwar, A;Kumar, S
目的：研究来自男性和绝经前和绝经后女性的人脂肪组织中P450芳香化酶活性（负责C19雄激素向C18雌激素的转化）的激素调节。2.5;平均体重76.01 ± 5.6 kg），8例绝经后女性（平均年龄59.9 +/-2.0;平均体重63.5 +/-2.6kg）和5名男性（平均年龄35.8 +/-8.8;平均体重78.5 +/-7.8kg）进行选择性或整容手术。用RT-PCR证实芳香化酶的存在，用雄烯二酮作为底物在氚水释放试验中表征芳香化酶活性的调节。在含有皮质醇（10（-6）-10（-7）M）、胰岛素（500 nM）或两者组合的无血清培养基中培养的腹部皮下基质细胞（ASC）和成熟脂肪细胞（AD）中分析芳香化酶活性。在ASC中，雌性芳香化酶活性从14.5 +/- 1.7增加到29.3 +/- 2.6 pmol/mg/h（n = 14，P < 0.05），皮质醇（10（-7）M）和胰岛素分别升高至25.2 ± 2.1 pmol/mg/h（P
OBJECTIVE: To investigate the hormonal regulation of P450 aromatase activity (responsible for the conversion of C19 androgens to C18 oestrogens) in human adipose tissue from men and pre- and post-menopausal women.SUBJECTS: Subcutaneous abdominal adipose tissue was obtained from 19 subjects: six pre-menopausal females (mean age 41.8 +/- (s.e.m.) 2.5; mean weight 76.01 +/- 5.6 kg), eight post-menopausal females (mean age 59.9 +/- 2.0; mean weight 63.5 +/- 2.6 kg), and five males (mean age 35.8 +/- 8.8; mean weight 78.5 +/- 7.8 kg) undergoing elective or cosmetic surgery.MEASUREMENTS: Cell viability and cell size were determined using staining techniques. RT-PCR was used to confirm the presence of aromatase, The regulation of aromatase activity was characterized using androstenedione as a substrate in a tritiated water release assay. Aromatase activity was analysed in abdominal subcutaneous stromal cells (ASC) and mature adipocytes (AD) cultured in serum-free medium with cortisol (10(-6)-10(-7) M), insulin (500 nM) or a combination of both.RESULTS: In ASC aromatase activity increased in females from 14.5 +/- 1.7 to 29.3 +/- 2.6 pmol/mg/h (n = 14, P < 0.05) and to 25.2 +/- 2.1 pmol/mg/h with cortisol (10(-7) M) and insulin, respectively (P