Isolation, sequence analysis, and characterization of androgen receptor in Southern catfish, Silurus meridionalis
Isolation, sequence analysis, and characterization of androgen receptor in Southern catfish, Silurus meridionalis
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南方鲶雄激素受体的分离、序列分析和表征
DOI：
10.1007/s10695-010-9461-0
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发表时间：
2011-09-01
影响因子：
2.9
通讯作者：
Wang, D. S.
中科院分区：
文献类型：
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作者：
Huang, B. F.;Sun, Y. L.;Wang, D. S.
雄激素受体（Androgen receptor，AR）是雄激素的介质，在雄激素信号通路中起重要作用.本研究分离并分析了南方鲶鱼雄激素受体（scAR）的cDNA序列及其组织分布。scAR基因全长cDNA为3,116 bp，开放阅读框（ORF）为2,415 bp，编码804个氨基酸。组织分布分析表明，scAR在所有组织中均有表达，在卵巢和睾丸中无性别二型性。系统进化分析和多氨基酸序列比对表明，scAR与鲤形目鱼类AR的亲缘关系较近，具有较高的相似性。此外，还从其他7个鲶形目物种中分离到了AR基因的部分序列。与其他脊椎动物的比较发现，鲶形目鱼类的ARs在C末端都多了一条约20个氨基酸的尾巴，而其他脊椎动物的ARs则没有。这一额外序列是由于在其它脊椎动物AR终止密码子前插入了一个4-bp的片段而产生的，并且在同属的鲇形目动物的AR中是相同的，而在不同属的物种的AR中是不同的。我们首次报道了鲇形鱼类AR的C末端比其他脊椎动物的AR更长，这可能有助于重建鲇形鱼类之间的系统发育关系。额外的C-末端尾部对AR功能的意义仍然难以捉摸。
Androgen receptor (AR), the mediator of androgen, plays important roles in the androgen signal pathway. In the present study, we isolated and analyzed the cDNA sequence and tissue distribution of androgen receptor in Southern catfish (scAR). The full-length cDNA of scAR contains 3,116 bp with an open reading frame (ORF) of 2,415 bp, encoding a protein of 804 amino acids (aa). Tissue distribution analysis of scAR revealed that it was expressed in all tissues examined, with no sexual dimorphism in the ovary and testis. Phylogenetic analysis and multiple amino acids sequence alignment indicated the close relationship and high similarity of scAR with ARs from cypriniform species. In addition, partial sequences of ARs from 7 other siluriform species were also isolated. Comparison of catfish ARs with those from other vertebrates revealed that an extra C-terminal tail of about 20aa exists in all the ARs from siluriform fishes investigated, but not in other ARs. The extra sequence was resulted from a 4-bp insertion before the stop codon of other vertebrate ARs, and it was identical in ARs from siluriform species of the same genus but different among ARs from species of different genera. We report here for the first time that the ARs from siluriform species are longer in C-terminal than those from other vertebrates and it might be useful in reconstruction of the phylogenetic relationship among siluriform fishes. The significance of the extra C-terminal tail for AR function remains elusive.