Biosensors for detection of mercury in contaminated soils
Biosensors for detection of mercury in contaminated soils
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DOI：
10.1016/j.envpol.2004.02.019
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发表时间：
2004-09-01
影响因子：
8.9
通讯作者：
Csöregi, E
中科院分区：
文献类型：
--
作者：
Bontidean, L;Mortari, A;Csöregi, E
采用细菌全细胞生物传感器和细菌重金属结合蛋白生物传感器测定土壤中汞的浓度。这些土壤样本是在25年前意外受到元素汞污染的一个菜园采集的。用不同的传感器：基于蛋白质的生物传感器、基于全细菌细胞的生物传感器和植物传感器来测量生物有效性汞，即在汞污染土壤中生长的菜豆幼苗的初生叶和根的形态和生化反应。为了进行比较，用原子吸收光谱法测定了土壤样品的总汞含量。整个细菌细胞和基于蛋白质的生物传感器给出了与土壤样品中汞总量成正比的准确响应。相反，植物传感器被发现不是土壤汞污染的有用指标，这是由植物生物量、初生叶的汞含量和酶活性决定的。(C)2004爱思唯尔有限公司。保留所有权利。
Biosensors based on whole bacterial cells and on bacterial heavy metal binding protein were used to determine the mercury concentration in soil. The soil samples were collected in a vegetable garden accidentally contaminated with elemental mercury 25 years earlier. Bioavailable mercury was measured using different sensors: a protein-based biosensor, a whole bacterial cell based biosensor, and a plant sensor, i.e. morphological and biochemical responses in primary leaves and roots of bean seedlings grown in the mercury-contaminated soil. For comparison the total mercury concentration of the soil samples was determined by AAS. Whole bacterial cell and protein-based biosensors gave accurate responses proportional to the total amount of mercury in the soil samples. On the contrary, plant sensors were found to be less useful indicators of soil mercury contamination, as determined by plant biomass, mercury content of primary leaves and enzyme activities. (C) 2004 Elsevier Ltd. All rights reserved.