Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures.
Fractions of lipopolysaccharide from Escherichia coli O111:B4 prepared by two extraction procedures.
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DOI：
10.1016/s0021-9258(19)41574-3
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发表时间：
1975-04
期刊：
影响因子：
--
通讯作者：
D. Morrison;L. Leive
中科院分区：
文献类型：
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作者：
D. Morrison;L. Leive
通过苯酚萃取和采用水性丁醇的新方法，从大肠杆菌 O111:B4 中提取了脂多糖。两种方法产生非常相似的脂多糖制剂。任一制剂的凝胶过滤色谱产生两种物理和化学上不同的脂多糖级分。一种组分含有具有长抗原侧链的脂多糖分子。它的作用就像一个高度不对称的单元，表观重量为 10-6 的 1.5 倍，并且不会因去污剂或脱酰作用而解离。第二部分具有短抗原侧链，可以通过十二烷基硫酸钠和 Triton X-100 解离成大约 90,000 个单位。脂多糖组分的一些特性随提取方法的不同而变化。
Lipopolysaccharides have been extracted from Escherichia coli O111:B4 by phenol extraction and by a new method employing aqueous butanol. Both methods yield very similar lipopolysaccharide preparations. Gel filtration chromatography of either preparation yields two physically and chemically distinct lipopolysaccharide fractions. One fraction contains lipopolysaccharide molecules with long antigenic side chains. It acts like a highly asymmetric unit with an apparent weight of 1.5 times 10-6 and is not dissociated by detergents or deacylation. The second fraction has a short antigenic side chain and can be dissociated by sodium dodecyl sulfate and Triton X-100 into units of approximately 90,000. Some properties of the lipopolysaccharide fractions vary with the method of extraction.