COSPECIATION ANALYSIS OF AN OBLIGATE POLLINATION MUTUALISM: HAVE GLOCHIDION TREES (EUPHORBIACEAE) AND POLLINATING EPICEPHALA MOTHS (GRACILLARIIDAE) DIVERSIFIED IN PARALLEL?
COSPECIATION ANALYSIS OF AN OBLIGATE POLLINATION MUTUALISM: HAVE GLOCHIDION TREES (EUPHORBIACEAE) AND POLLINATING EPICEPHALA MOTHS (GRACILLARIIDAE) DIVERSIFIED IN PARALLEL?
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DOI：
10.1554/04-187
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
A. Kawakita;Atsushi Takimura;T. Terachi;T. Sota;Makoto Kato
中科院分区：
文献类型：
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作者：
A. Kawakita;Atsushi Takimura;T. Terachi;T. Sota;Makoto Kato
Glochidion树(大戟科)和表头蛾(Gracillariidae)之间的专性授粉互惠关系涉及大量相互作用的物种，类似于经典的无花果-无花果黄蜂和丝兰-丝兰蛾的群丛。为了评估Glochidion-Eicephala组合中平行分支发生的程度，我们使用核糖体DNA序列(内部和外部转录间隔区)重建了18种Glochidion的系统发育关系，并使用线粒体(细胞色素氧化酶亚基I基因)和核DNA序列(精氨酸激酶和延伸因子-1a基因)重建了相应18种Eicephala物种的系统发育关系。基于所获得的系统发育，我们使用几种不同的方法来研究系统发育之间的同种程度，以确定Glochidion和Eicephala是否经历了平行的多样化。这些检验表明，在随机组合中，Glochidion和Eicephala的系统发育之间的相关性通常比预期的要大，但结果对评估共种关系的不同系统发育假说和分析方法的选择是敏感的。在这种联系中没有发现系统发育之间的完全一致，这可能是由于寄主被蛾转移所致。所观察到的Glochidion和Eicephala系统发育的显著差异表明，植物和蛾之间的一对一的特异性通过复杂的物种形成过程保持着，或者是Glochidion树和Eicephala蛾之间的联系的多样性被低估了。
Species-specific obligate pollination mutualism between Glochidion trees (Euphorbiaceae) and Epicephala moths (Gracillariidae) involves a large number of interacting species and resembles the classically known fig–fig wasp and yucca–yucca moth associations. To assess the extent of parallel cladogenesis in Glochidion-Epicephala association, we reconstruct phylogenetic relationships of 18 species of Glochidion using nuclear ribosomal DNA sequences (internal and external transcribed spacers) and those of the corresponding 18 Epicephala species using mitochondrial (the cytochrome oxidase subunit I gene) and nuclear DNA sequences (the arginine kinase and elongation factor-1a genes). Based on the obtained phylogenies, we determine whether Glochidion and Epicephala have undergone parallel diversification using several different methods for investigating the level of cospeciation between phylogenies. These tests indicate that there is generally a greater degree of correlation between Glochidion and Epicephala phylogenies than expected in a random association, but the results are sensitive to selection of different phylogenetic hypotheses and analytical methods for evaluating cospeciation. Perfect congruence between phylogenies is not found in this association, which likely resulted from host shift by the moths. The observed significant discrepancy between Glochidion and Epicephala phylogenies implies that the one-to-one specificity between the plants and moths has been maintained through a complex speciation process or that there is an underestimated diversity of association between Glochidion trees and Epicephala moths.