Cutting edge: Memory B cell survival and function in the absence of secreted antibody and immune complexes on follicular dendritic cells
Cutting edge: Memory B cell survival and function in the absence of secreted antibody and immune complexes on follicular dendritic cells
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DOI：
10.4049/jimmunol.176.8.4515
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发表时间：
2006-04-15
影响因子：
4.4
通讯作者：
Shlomchik, Mark J.
中科院分区：
文献类型：
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作者：
Anderson, Shannon M.;Hannum, Lynn G.;Shlomchik, Mark J.
Ag以免疫复合物的形式保留在滤泡树突状细胞上，与B细胞记忆的发育和维持有关。我们使用缺乏分泌型IG（mIG）的H链转基因（Tg）小鼠模型解决了这个问题，因此不会存款含Ag的免疫复合物。我们比较了mIg菌株和分泌IgM的对照Tg菌株开发和维持长寿记忆细胞的能力。免疫后，有一个增加的Ag-特异性B细胞在两个菌株，维持至少20周。我们用BrdU标记了长寿命的Ag特异性细胞，发现这一群体也得到了类似的维持。此外，这两个TGS都能够保持功能性记忆反应所测量的次级germinal中心的反应。我们的研究表明，定位的Ag滤泡树突状细胞是不必要的发展和维持B细胞的记忆。
Ag, in the form of immune complexes retained on follicular dendritic cells, has been implicated in the development and maintenance of B cell memory. We addressed this question using a H chain transgenic (Tg) mouse model that lacks secreted Ig (mIg), and thus does not deposit Ag-containing immune complexes. We compared the ability of the mIg strain and a control Tg strain, which secretes IgM, to develop and maintain long-lived memory cells. After immunization, there was an increase of Ag-specific B cells in both strains that was maintained for at least 20 wk. We labeled the long-livedAg-specific cells with BrdU and found that this population was similarly maintained. In addition, both Tgs were able to maintain a functional memory response as measured by secondary germinal center reactions. Our studies indicate that localization of Ag on follicular dendritic cells is not necessary for development and maintenance of B cell memory.