Identification of DNA basepairing via tunnel-current decay
Identification of DNA basepairing via tunnel-current decay
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DOI：
10.1021/nl0726205
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发表时间：
2007-12-01
期刊：
影响因子：
10.8
通讯作者：
Lindsay, Stuart
中科院分区：
文献类型：
--
作者：
He, Jin;Lin, Lisha;Lindsay, Stuart
我们提出了一种新的方法来读取DNA分子通过纳米孔电极之间的序列，使用氢键介导的隧道信号。使用从核苷单分子膜上拉出的核碱基功能化的STM探针来模拟碱基-电极的相互作用。沃森-克里克的识别导致了隧道电流的缓慢衰减，这是超过一半的读数中基准线的独特特征。13个独立的读数将产生所需的99.99%的准确率。
We propose a new approach for reading the sequence of a DNA molecule passing between electrodes on a nanopore, using hydrogen bond-mediated tunneling signals. The base-electrode interaction is modeled using a nucleo-base-functionalized STM probe that is pulled away from a nucleoside monolayer. Watson-Crick recognition results in slow decay of the tunnel current, which is uniquely characteristic of the basepair in over half the reads. Thirteen independent reads would yield the desired 99.99% accuracy.