Microencapsulated olfactory ensheathing-cell transplantation reduces pain in rats by inhibiting P2X4 receptor overexpression in the dorsal root ganglion
Microencapsulated olfactory ensheathing-cell transplantation reduces pain in rats by inhibiting P2X4 receptor overexpression in the dorsal root ganglion
复制标题
微囊嗅鞘细胞移植通过抑制背根神经节 P2X4 受体过度表达减轻大鼠疼痛
DOI：
10.1097/wnr.0000000000001170
复制
发表时间：
2019-01-16
期刊：
影响因子：
1.7
通讯作者：
Yu, Qing
中科院分区：
文献类型：
--
作者：
Zhang, Wenjun;Liu, Yuguo;Yu, Qing
本研究旨在探讨微囊化嗅鞘细胞移植（MC-OEC）对坐骨神经损伤致痛大鼠的作用及其与背根神经节P2 X4受体表达水平的关系。取健康SD大鼠的嗅球组织，采用差速贴壁法培养嗅鞘细胞。将96只健康SD大鼠随机分为假手术组、慢性压迫性损伤（CCI）组、嗅鞘细胞（OEC）组和MC-OEC组。在手术后7天和14天测量机械缩爪阈值。采用逆转录聚合酶链反应、荧光原位杂交和蛋白质印迹法检测L4-5背根神经节P2 X4受体基因的表达。术后第7天和第14天，MC-OEC、OEC和CCI组大鼠的机械缩爪阈值均低于假手术组。CCI组、OEC组和MC-OEC组L4-5背根神经节P2 X4受体表达水平均高于假手术组。组间的所有差异均具有统计学显著性。OEC和MC-OEC移植可减轻神经病理性疼痛并抑制L4-5背根神经节P2 X4受体的过度表达。MC-OEC组移植MC-OEC的效果优于OEC组。
The aim of this study was to determine the role of microencapsulated olfactory ensheathing-cell transplantation (MC-OEC) in rats in which pain was induced by sciatic nerve injury, and its relationship with the expression level of the P2X4 receptor in the dorsal root ganglion. Olfactory bulb tissues of healthy Sprague-Dawley rats were collected to culture olfactory ensheathing cells using differential attachment methods. Ninety-six healthy Sprague-Dawley rats were randomly assigned to the sham, chronic constriction injury (CCI), olfactory ensheathing cell (OEC), and MC-OEC groups. Mechanical paw withdrawal thresholds were measured 7 and 14 days after surgery. The expression of P2X4 receptor genes in the L4-5 dorsal root ganglion was detected by reverse transcriptase polymerase chain reaction, fluorescence in-situ hybridization, and western blotting. Seven and 14 days after the surgery, the mechanical paw withdrawal thresholds of rats in the MC-OEC, OEC, and CCI groups were decreased compared with the sham group. The expression level of the P2X4 receptor in the L4-5 dorsal root ganglion in CCI, OEC, and MC-OEC groups was increased compared with the sham group. All differences between groups were statistically significant. Transplantation of OEC and MC-OEC can reduce neuropathic pain and inhibit the overexpression of the P2X4 receptor in the L4-5 dorsal root ganglion. The transplantation of MC-OEC was more effective in the MC-OEC group than in the OEC group.