Both Subunits of the Circadian RNA-Binding Protein CHLAMY1 Can Integrate Temperature Information1[W]
Both Subunits of the Circadian RNA-Binding Protein CHLAMY1 Can Integrate Temperature Information1[W]
复制标题
昼夜节律 RNA 结合蛋白 CHLAMY1 的两个亚基都可以整合温度信息1[W]
作者：
Olga Voytsekh;S. Seitz;Dobromir Iliev;M. Mittag
来自绿色莱茵衣藻的昼夜节律RNA结合蛋白CHLAMY 1由命名为C1和C3的两个亚基组成。C1水平的变化导致趋光性节律的重复性，而C3水平的改变导致顶相位移。因此，CHLAMY 1参与维持生物钟的周期和相位。在这里，我们分析了这两个亚基在整合温度信息中的作用，这是生物钟其他关键特性的基础，包括温度周期的夹带和温度补偿。施加的温度（18°C和28°C）在生理范围C内。莱因哈德氏菌虽然C1在低温下过度磷酸化，但C3表达水平在18°C下上调。抑制剂实验表明，这种上调发生在转录水平。启动子分析研究沿着单个启动子元件突变显示，单独替换两个DREB 1A盒降低了18°C时c3上调的幅度，而替换一个E盒则完全消除了c3上调。E-box的替换也引起了昼夜节律控制的c3表达的不稳定性。因此，E盒具有c3的温度依赖性上调以及其昼夜节律表达的双重功能。我们还发现，C1和C3的温度依赖性调节以及温度夹带在时钟突变体per 1中改变，表明C1，C3和PER 1的温度控制网络存在。
The circadian RNA-binding protein CHLAMY1 from the green alga Chlamydomonas reinhardtii consists of two subunits named C1 and C3. Changes in the C1 level cause arrhythmicity of the phototaxis rhythm, while alterations in the level of C3 lead to acrophase shifts. Thus, CHLAMY1 is involved in maintaining period and phase of the circadian clock. Here, we analyzed the roles of the two subunits in the integration of temperature information, the basis for other key properties of circadian clocks, including entrainment by temperature cycles and temperature compensation. Applied temperatures (18°C and 28°C) were in the physiological range of C. reinhardtii. While C1 is hyperphosphorylated at low temperature, the C3 expression level is up-regulated at 18°C. An inhibitor experiment showed that this up-regulation occurs at the transcriptional level. Promoter analysis studies along with single promoter element mutations revealed that individual replacement of two DREB1A-boxes lowered the amplitude of c3 up-regulation at 18°C, while replacement of an E-box abolished it completely. Replacement of the E-box also caused arrhythmicity of circadian-controlled c3 expression. Thus, the E-box has a dual function for temperature-dependent up-regulation of c3 as well as for its circadian expression. We also found that the temperature-dependent regulation of C1 and C3 as well as temperature entrainment are altered in the clock mutant per1, indicating that a temperature-controlled network of C1, C3, and PER1 exists.
影响因子：
3.5
作者：
HORTON, RM;HUNT, HD;PEASE, LR
通讯作者：
PEASE, LR
影响因子：
10.5
作者：
Yu, WJ;Zheng, H;Hardin, PE
通讯作者：
Hardin, PE
DOI：
10.1073/pnas.90.12.5672
发表时间：
1993-06-15
影响因子：
11.1
作者：
KONDO, T;STRAYER, CA;JOHNSON, CH
通讯作者：
JOHNSON, CH