MITOGENIC, CHEMOTACTIC, AND SYNTHETIC RESPONSES OF RAT PERIODONTAL-LIGAMENT FIBROBLASTIC CELLS TO POLYPEPTIDE GROWTH-FACTORS INVITRO
MITOGENIC, CHEMOTACTIC, AND SYNTHETIC RESPONSES OF RAT PERIODONTAL-LIGAMENT FIBROBLASTIC CELLS TO POLYPEPTIDE GROWTH-FACTORS INVITRO
复制标题
DOI：
10.1902/jop.1992.63.6.515
复制
发表时间：
1992-06-01
影响因子：
4.3
通讯作者：
GENCO, RJ
中科院分区：
文献类型：
--
作者：
MATSUDA, N;LIN, WL;GENCO, RJ
体外培养大鼠牙周膜成纤维细胞，观察其对表皮生长因子(EGF)、转化生长因子-β(TGF-β)、重组人血小板衍生生长因子(rhPDGF-BB)、天然(N)PDGF-AB和胰岛素样生长因子-I(IGF-I)的促有丝分裂、趋化和合成反应。PDGFs和IGF-I对PDL成纤维细胞有较强的促有丝分裂作用。PDGFs的促有丝分裂作用在浓度为10 ng/ml时达到最大，而IGF-I的促分裂作用在100 ng/ml以上时达到最大，相反，EGF可诱导中等程度的和转化生长因子-β抑制的促分裂反应。无论其他生长因子的促有丝分裂作用如何，重组人PDGF-AB与EGF或转化生长因子-β均显示出类似的促有丝分裂能力，相当于PDGF单独作用的水平。然而，在所有单独的生长因子以及任何生长因子的组合中，重组人PDGF-AB和IGF-I的组合显示出协同作用，显示出最高的促分裂作用。类似地，PDL成纤维细胞对IGF-I和PDGFs均表现出强烈的趋化反应。IGF-I浓度大于10 ng/ml时作用最强，其次是0.1 ng/ml的重组人PDGF-BB、0.1~1 ng/ml浓度的重组人PDGF-AB和nPDGF，转化生长因子β表达NO，EGF轻度升高，具有趋化作用。IGF-I对总蛋白合成略有促进作用，而其他因素对总蛋白合成无明显影响。然而，重组人PDGF-AB和转化生长因子-β都能促进胶原合成。另一方面，IGF-I对胶原合成无影响，而EGF则抑制胶原合成。这些结果提示，重组人PDGF-BB和IGF-I可刺激PDL成纤维细胞的增殖和趋化。此外，这些生长因子的组合进一步增加了有丝分裂的作用。重组人PDGF-AB还能刺激PDL成纤维细胞合成胶原。因此，重组人PDGF-BB和IGF-I可能在促进PDL愈合方面发挥重要作用，因此，可能有助于在牙周再生手术中的临床应用。
THE MITOGENIC, CHEMOTACTIC, AND SYNTHETIC RESPONSES of rat periodontal ligament (PDL) fibroblastic cells to epidermal growth factor (EGF), transforming growth factor-beta (TGF-beta), recombinant human platelet-derived growth factor (rhPDGF)-AB, rhPDGF-BB, natural (n) PDGF-AB, and insulin-like growth factor-I (IGF-I) were examined in vitro using PDL cells obtained from the coagulum of healing tooth sockets. PDGFs and IGF-I have potent and comparable mitogenic effects on PDL fibroblastic cells. The maximum mitogenic effect of PDGFs was observed at the concentration of 10 ng/ml, whereas that of IGF-I was seen at concentrations higher than 100 ng/ml. In contrast, EGF induced moderate, and TGF-beta inhibitory mitogenic responses. The combination of rhPDGF-AB with either EGF or TGF-beta demonstrated comparable mitogenic potency, equivalent to the level of PDGF alone regardless of the mitogenic effect of other growth factors. The combination of rhPDGF-AB and IGF-I, however, showed a synergistic effect revealing the highest mitogenic effect among all individual growth factors as well as any combinations of the growth factors tested. Similarly, PDL fibroblastic cells demonstrated strong chemotactic responses to both IGF-I and PDGFs. The maximum effect was observed by IGF-I at concentrations higher than 10 ng/ml, followed by rhPDGF-BB at 0.1 ng/ml, rhPDGF-AB and nPDGF at concentrations ranging from 0.1 to 1 ng/ml. TGF-beta revealed no, and EGF slightly increased, chemotactic effects. IGF-I slightly enhanced the synthesis of total protein, whereas other factors had no significant effect. However, both rhPDGF-AB and TGF-beta stimulated collagen synthesis. On the other hand, IGF-I showed no effect on collagen synthesis, while EGF suppressed collagen synthesis. These findings suggest that rhPDGF-BB and IGF-I stimulate proliferation and chemotaxis of PDL fibroblastic cells. In addition, the combination of these growth factors further increases the mitogenic effect. rhPDGF-AB also stimulates collagen synthesis by PDL fibroblastic cells. Thus, rhPDGF-BB and IGF-I may have important roles in promotion of PDL healing, and consequently, may be useful for clinical application in periodontal regenerative procedures.