SCID MOUSE MODELS OF ACUTE AND RELAPSING CHRONIC TOXOPLASMA-GONDII INFECTIONS
SCID MOUSE MODELS OF ACUTE AND RELAPSING CHRONIC TOXOPLASMA-GONDII INFECTIONS
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DOI：
10.1128/iai.60.9.3719-3724.1992
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发表时间：
1992-09-01
影响因子：
3.1
通讯作者：
JOHNSON, LL
中科院分区：
文献类型：
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作者：
JOHNSON, LL
淋巴缺陷型SCID/SCID(SCID)小鼠死于急性感染弓形虫株，导致免疫功能正常的非SCID小鼠慢性感染，症状轻微。然而，大多数SCID小鼠在感染弓形虫前一个月用免疫活性小鼠的脾细胞重组，在出现疾病的短暂时期后健康状况良好。未重组的SCID小鼠从弓形虫感染第10天起在饮用水中给予磺胺嘧啶，可存活到感染急性期，并存活数周，没有明显症状。组织学检查显示，他们的大脑中有弓形虫包囊。然而，如果从这些慢性感染的SCID小鼠的饮用水中停用磺胺嘧啶，这些小鼠在1周内死亡，脑中充满大量滋养体。这些发现使SCID小鼠成为一种潜在的有用资源，用来研究弓形虫感染在急性或慢性阶段的免疫控制的各个方面。此外，在SCID小鼠中产生无毒弓形虫慢性感染和随意引起急性复发感染的能力表明，SCID小鼠可能有助于评估免疫低下患者的急性和慢性弓形虫感染的潜在治疗方法。
Lymphodeficient scid/scid (SCID) mice died from acute infection with a strain of Toxoplasma gondii that causes chronic infection with mild symptoms in immunocompetent non-SCID mice. However, most SCID mice reconstituted with spleen cells from immunocompetent mice 1 month prior to T. gondii infection survived in good health after a transient period during which they appeared ill. Unreconstituted SCID mice given sulfadiazine in their drinking water from day 10 of Toxoplasma infection onward survived the acute phase of infection and lived for many weeks without overt symptoms. Histological examination revealed Toxoplasma cysts in their brains. However, if sulfadiazine was withdrawn from the drinking water of these chronically infected SCID mice, the mice died within 1 week with large numbers of trophozoites throughout their brains. These findings establish SCID mice as a potentially useful resource with which to study various aspects of immunological control of T. gondii infection during either its acute or chronic phase. Furthermore, the ability to produce chronic infections with avirulent T. gondii in SCID mice and to cause acute relapsing infections at will suggests that SCID mice may be helpful in evaluating potential therapies for acute and chronic T. gondii infections in immunocompromised patients.