Tissue localization and fate in mice of injected multipotential colony-stimulating factor.
Tissue localization and fate in mice of injected multipotential colony-stimulating factor.
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注射多能集落刺激因子的小鼠的组织定位和命运。
DOI：
10.1073/pnas.85.9.3160
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发表时间：
1988
影响因子：
11.1
通讯作者：
Nicola,NA
中科院分区：
文献类型：
--
作者：
Metcalf,D;Nicola,NA
造血调节因子多能集落刺激因子[Multi-CSF（白细胞介素3）]在体外和体内对多种造血细胞具有增殖作用。天然或重组Multi-CSF静脉注射到成年小鼠中的初始半衰期为3-5 min，第二相为50 min。在静脉注射重组125 I标记的Multi-CSF的小鼠的骨髓和脾脏中观察到造血细胞的清晰标记，表明注射的Multi-CSF可以原位获得这些细胞。注射的两种类型的125 I标记的Multi-CSF的高比例定位于肝脏和肾脏（Bowman囊和近端肾小管的细胞中）。肾脏似乎是Multi-CSF降解的活性部位，尿液中早期出现低分子量标记物质。
The hemopoietic regulator multipotential colony-stimulating factor [Multi-CSF (interleukin 3)] has proliferative effects on a wide range of hemopoietic cells in vitro and in vivo. Native or recombinant Multi-CSF injected intravenously into adult mice had an initial half-life of 3-5 min and a second phase of 50 min. Clear labeling of hemopoietic cells was observed in the bone marrow and spleen of mice injected intravenously with recombinant 125I-labeled Multi-CSF showing that injected Multi-CSF can obtain access to such cells in situ. A high proportion of injected 125I-labeled Multi-CSF of both types became localized in the liver and in the kidney (in cells of the Bowman's capsule and proximal renal tubules). The kidney appeared to be an active site of degradation of Multi-CSF with the early appearance of low molecular weight labeled material in the urine.