A functional link between RuBisCO-like protein of Bacillus and photosynthetic RuBisCO
A functional link between RuBisCO-like protein of Bacillus and photosynthetic RuBisCO
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DOI：
10.1126/science.1086997
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发表时间：
2003-10-10
期刊：
影响因子：
56.9
通讯作者：
Yokota, A
中科院分区：
文献类型：
--
作者：
Ashida, H;Saito, Y;Yokota, A
一些非光合细菌（例如枯草芽孢杆菌）和一些古细菌的基因组包含与核酮糖二磷酸羧化酶/加氧酶（RuBisCO）大亚基具有序列同源性的蛋白质基因。我们发现来自枯草芽孢杆菌的 RuBisCO 样蛋白 (RLP) 催化甲硫氨酸补救途径中的 2,3-二酮-5-甲基硫代戊基-1-磷酸烯醇酶反应。一种生长缺陷型突变体，其中 RLP 基因被破坏，被来自光合细菌红色红螺菌的 RuBisCO 基因拯救。因此，来自 R. rubrum 的光合 RuBisCO 保留了在枯草芽孢杆菌中的蛋氨酸补救途径中发挥作用的能力。
The genomes of several nonphotosynthetic bacteria, such as Bacillus subtilis, and some Archaea include genes for proteins with sequence homology to the large subunit of ribulose bisphosphate carboxylase/oxygenase (RuBisCO). We found that such a RuBisCO-like protein (RLP) from B. subtilis catalyzed the 2,3-diketo-5-methylthiopentyl-1-phosphate enolase reaction in the methionine salvage pathway. A growth-defective mutant, in which the gene for this RLP had been disrupted, was rescued by the gene for RuBisCO from the photosynthetic bacterium Rhodospirillum rubrum. Thus, the photosynthetic RuBisCO from R. rubrum retains the ability to function in the methionine salvage pathway in B. subtilis.