Simultaneous determination of ginkgo flavonoids and terpenoids in plasma: Ammonium formate in LC mobile phase enhancing electrospray ionization efficiency and capacity
Simultaneous determination of ginkgo flavonoids and terpenoids in plasma: Ammonium formate in LC mobile phase enhancing electrospray ionization efficiency and capacity
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DOI：
10.1016/j.jasms.2007.11.015
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发表时间：
2008-03
影响因子：
3.2
通讯作者：
Yang Zhao;Yan Sun;Chuan Li
中科院分区：
文献类型：
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作者：
Yang Zhao;Yan Sun;Chuan Li
银杏叶提取物通过类黄酮和萜类化合物的协同作用发挥其治疗作用。我们在此描述了一种基于LC-MS/MS的方法，用于同时测定酸水解血浆中的黄酮类化合物（槲皮素、山奈酚和异鼠李素）和萜类化合物（白果内酯、银杏内酯A、B、C和J），该方法通过避免所鉴定的黄酮类化合物和萜类化合物之间的交叉干扰。值得注意的是，在移动的相中加入甲酸铵（0.2 mM）产生了有益的LC-电解质效应，包括增加的ESI效率和容量，结果是新开发的方法显示出迄今为止银杏相关研究报告的最高分析性能。该方法具有高灵敏度、可忽略的基质干扰和交叉干扰、宽线性动态范围、高样品通量和相当小的初始样品量。对商业银杏产品的狗药代动力学测量的测定效用产生了迄今为止关于银杏化合物的全身暴露的最全面的数据。新开发的多分析物程序应广泛使用。
Extracts fromGinkgo bilobaleaves confer their therapeutic effects through the synergistic actions of flavonoid and terpenoid components. We herein describe the development of an LC-MS/MS-based method for simultaneous determination of flavonoids (quercetin, kaempferol, and isorhamnetin) and terpenoids (bilobalide, ginkgolides A, B, C, and J) in acid-hydrolyzed plasma by circumventing cross-interference between the flavonoids and terpenoids identified. Notably, inclusion of ammonium formate (0.2 mM) in the mobile phase generated beneficial LC-electrolyte effects, including increased ESI efficiency and capacity, with the result that the newly developed procedure exhibits the highest analytical performance reported to date for ginkgo-associated studies. The method yields high sensitivity, negligible matrix interference and cross-interference, wide linear dynamic ranges, high sample throughput, and quite small initial sample size. The assay utility to dog pharmacokinetic measurements of commercial ginkgo products yielded the most comprehensive data on systemic exposure to the ginkgo compounds to date. The newly developed multi-analyte procedure should be widely useful.