The existence of 25-hydroxyvitamin D3-1α-hydroxylase in the liver of carp and bastard halibut
The existence of 25-hydroxyvitamin D3-1α-hydroxylase in the liver of carp and bastard halibut
复制标题
鲤鱼和大比目鱼肝脏中25-羟基维生素D3-1α-羟化酶的存在
DOI：
10.1016/0024-3205(91)90355-f
复制
发表时间：
1991
期刊：
影响因子：
6.1
通讯作者：
Tadashi Kobayashi
中科院分区：
文献类型：
--
作者：
A. Takeuchi;T. Okano;Tadashi Kobayashi
通过体内和体外实验，我们发现鲤鱼和大比目鱼的肝脏中含有25-羟维生素D3（25-D3）-1α-羟化酶。将[3 H]-25-D3注射到维生素D缺乏的鲤鱼和正常的比目鱼（缺乏维生素D的比目鱼在养殖过程中死亡而无法饲养）的血浆中，其血浆脂质提取物的高效液相色谱（HPLC）图谱显示形成了对应于[3 H]-1 α，25-二羟基维生素D3（1，25-D3）的峰。当[~ 3 H]-25-D3与鱼肝匀浆孵育时，在HPLC图谱中也观察到对应于[~ 3 H]-1，25-D3的峰。通过热异构化为前体异构体和质量碎片分析法证实了代谢产物的形成。虽然在肾脏中也观察到1 α-羟化酶，但该酶的活性低于肝脏。结果表明，鲤鱼和大比目鱼肝脏中存在25-D3-1 α-羟化酶，肝脏中D3转化为25-D3后，在同一组织中迅速代谢为1，25-D3。D3是一种主要的循环化合物，在鱼体内含有少量的1，25-D3，而血浆中25-D3的水平低于检测限，这一事实支持了这一建议。
We have found that carp and bastard halibut contain 25-hydroxyvitamin D3(25-D3)-1α-hydroxylase in the liver besides in the kidney by the followingin vivoandin vitroexperiments. When [3H]-25-D3was intraperitoneally injected to vitamin D(D)-deficient carp and normal bastard halibut (D-deficient bastard halibut could not be raised because they died during farming), the profiles of high-performance liquid chromatography (HPLC) of the plasma lipid extract showed the formation of a peak corresponding to [3H]-1 α, 25-dihydroxyvitamin D3(1, 25-D3). When [3H]-25-D3was incubated with liver homogenates of the fish, a peak corresponding to [3H]-1, 25-D3was also observed in the profile of HPLC. The formation of the metabolite was confirmed by the thermal isomerization into the pre-isomer and mass fragmentography. Although the 1 α-hydroxylase was also observed in the kidney, the activity of the enzyme was lower than that in the liver. The results suggest that 25-D3-1 α-hydroxylase exists in the liver of carp and bastard halibut and the 25-D3formed from D3in the liver is immediately metabolized into 1, 25-D3in the same tissue. The suggestion is supported by the fact that D3is a major circulating compound with small amounts of 1, 25-D3in the fish while the plasma levels of 25-D3are under the limit of detection.