Expression and Clinical Significance of lncRNA OSER1-AS1 in Peripheral Blood of Patients with Non-Small Cell Lung Cancer
Expression and Clinical Significance of lncRNA OSER1-AS1 in Peripheral Blood of Patients with Non-Small Cell Lung Cancer
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DOI：
10.1159/000519529
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发表时间：
2022-09-01
影响因子：
2.7
通讯作者：
Diao, Mingqiang
中科院分区：
文献类型：
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作者：
Chen, Peirui;Qin, Zheng;Diao, Mingqiang
非小细胞肺癌（NSCLC）是一种全球性的恶性癌症。长链非编码RNA（lncRNA）在NSCLC的发生、发展中起着重要作用，可能是NSCLC的潜在生物标志物。本研究旨在探讨非小细胞肺癌患者外周血中lncRNA氧化应激反应性丝氨酸1型反义RNA 1（OSER 1-AS 1）表达的临床意义。检测肺鳞癌（LUSC）、肺腺癌（LUAD）患者及健康人外周血中OSER1-AS1的表达，分析其临床诊断效能。分析OSER1-AS1表达与LUSC和LUAD患者临床病理特征的相关性。探讨了OSER1-AS1的下游作用机制。使用MedCalc分析比较LUSC和LUAD中lncRNA OSER 1-AS 1和miR-1298 - 5p/CHSY 3的ROC曲线下面积。OSER 1-AS 1在LUSC和LUAD患者外周血中表达较低。预测LUSC的ROC曲线下面积为0.800。预测LUAD的ROC曲线下面积为0.728。在LUSC和LUAD中，OSER 1-AS 1缺陷与肿瘤的淋巴结转移分期、淋巴结转移、远端转移和不良预后相关。miR-1298 - 5p在LUSC和LUAD患者中高表达，而硫酸软骨素合成酶3（CHSY 3）低表达。miR-1298 - 5p与OSER1-AS1和CHSY 3具有靶向关系。lncRNA OSER1-AS1在NSCLC患者中的诊断价值高于miR-1298 - 5p和CHSY3。总体而言，OSER1-AS1在NSCLC患者外周血中的低表达具有较高的临床意义，为NSCLC的早期诊断提供了一定的参考价值。
Non-small cell lung cancer (NSCLC) is a malignant cancer worldwide. Long non-coding RNAs (lncRNAs) have emerged as key players in the development and progression of NSCLC, and may be potential biomarkers of NSCLC. Here, we investigated the clinical significance of lncRNA oxidative stress responsive serine rich 1 antisense RNA 1 (OSER1-AS1) in peripheral blood of patients with NSCLC. OSER1-AS1 in peripheral blood of patients with lung squamous cell carcinoma (LUSC), lung adenocarcinoma (LUAD), and healthy subjects was detected, and the clinical diagnostic efficacy was analyzed. The correlation between OSER1-AS1 expression and clinicopathological features in patients with LUSC and LUAD was analyzed. The downstream mechanism of OSER1-AS1 was explored. The area under the ROC curve of lncRNA OSER1-AS1 and miR-1298-5p/CHSY3 in LUSC and LUAD was compared using the MedCalc analysis. OSER1-AS1 was low in peripheral blood of patients with LUSC and LUAD. The area under the ROC curve for predicting LUSC was 0.800. The area under the ROC curve for predicting LUAD was 0.728. In LUSC and LUAD, OSER1-AS1 deficiency was associated with tumor node metastasis stage, lymph node metastasis, distal metastasis, and poor prognosis. miR-1298-5p was highly expressed, while chondroitin sulfate synthase 3 (CHSY3) was lowly expressed in patients with LUSC and LUAD. miR-1298-5p had target relations with OSER1-AS1 and CHSY3. lncRNA OSER1-AS1 had a higher diagnostic value in patients with NSCLC than miR-1298-5p and CHSY3. Overall, low expression of OSER1-AS1 in peripheral blood of NSCLC patients has high clinical significance, which provides a certain reference value for NSCLC early diagnosis.