Amino acids: Chemistry, functionality and selected non-enzymatic post-translational modifications
Amino acids: Chemistry, functionality and selected non-enzymatic post-translational modifications
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DOI：
10.1016/j.jprot.2012.01.041
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发表时间：
2012-04-18
影响因子：
3.3
通讯作者：
Schlueter, Hartmut
中科院分区：
文献类型：
--
作者：
Bischoff, Rainer;Schlueter, Hartmut
蛋白质组学的最终目标是确定蛋白质物种的确切化学组成，包括它们的完整氨基酸序列和每个修饰侧链的鉴定，以及生物样品中每种蛋白质的定量。由于分析方法和数据分析的局限性，我们离实现这一目标还有很长的路要走，而且我们肯定还没有发现自然界中发生的所有氨基酸修饰。为了检测修饰侧链和发现新的、未知的氨基酸衍生物，必须了解氨基酸反应基团的化学性质。本教程的重点是氨基酸侧链的化学和地址的非酶修饰。通过突出一些示范反应的巨大多样性的氨基酸修饰的一瞥提供了读者对氨基酸化学有足够的洞察力，以提高对意想不到的侧链修饰的认识。我们进一步向读者介绍一个术语，它能够全面描述蛋白质物种的确切化学组成，包括其完整的氨基酸序列和其氨基酸侧链的所有修饰。本教程是国际蛋白质组学教程计划（IPTP第10号）的一部分。(C) 2012 Elsevier B.V.版权所有
The ultimate goal of proteomics is determination of the exact chemical composition of protein species, including their complete amino acid sequence and the identification of each modified side chain, in every protein in a biological sample and their quantification. We are still far from achieving this goal due to limitations in analytical methodology and data analysis but also due to the fact that we surely have not discovered all amino acid modifications that occur in nature. To detect modified side chains and to discover new, still unknown amino acid derivatives, an understanding of the chemistry of the reactive groups of amino acids is mandatory. This tutorial focuses on the chemistry of the amino acid side chains and addresses non-enzymatic modifications. By highlighting some exemplary reactions a glimpse of the huge diversity of modified amino acids provides the reader with sufficient insight into amino acid chemistry to raise the awareness for unexpected side chain modifications. We further introduce the reader to a terminology, which enables the comprehensive description of the exact chemical composition of a protein species, including its full amino acid sequence and all modifications of its amino acid side chains. This Tutorial is part of the International Proteomics Tutorial Programme (IPTP number 10). (C) 2012 Elsevier B.V. All rights reserved.