Dynamic Expression of Calcium-Regulatory Molecules, TRPV6 and S100G, in the Uterine Endometrium During Pregnancy in Pigs
Dynamic Expression of Calcium-Regulatory Molecules, TRPV6 and S100G, in the Uterine Endometrium During Pregnancy in Pigs
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DOI：
10.1095/biolreprod.109.076703
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发表时间：
2009-12-01
影响因子：
3.6
通讯作者：
Ka, Hakhyun
中科院分区：
文献类型：
--
作者：
Choi, Yohan;Seo, Heewon;Ka, Hakhyun
钙离子与妊娠的建立和维持有关，但在猪子宫内膜和受孕过程中钙离子的调节机制尚不清楚。最近，我们发现与钙离子进入细胞相关的钙离子通道蛋白TRPV6在猪着床期子宫内膜中高表达。在本研究中，我们研究了TRPV6和细胞内钙调节分子S100G在猪发情周期和妊娠期间子宫内膜的时空表达和调控。妊娠期子宫内膜TRPV6表达水平明显高于发情期。妊娠期TRPV6转录本和蛋白主要定位于腔上皮细胞（LE），弱定位于腺上皮细胞（GE）和绒毛膜（CM）。在妊娠第12天和第15天也检测到TRPV6的表达。雌激素治疗可提高子宫内膜TRPV6 mRNA水平。S100G的表达在妊娠第12天和妊娠第60天至足月呈双相增加，且主要局限于妊娠早期的LE和妊娠第30天至足月的LE、GE和CM。上述结果提示，妊娠期子宫内膜中TRPV6和S100G的时空表达受到动态调控，子宫内膜中TRPV6和S100G对钙离子浓度的调节可能对猪妊娠的建立和维持至关重要。
Calcium ions have been implicated in the establishment and maintenance of pregnancy, but the regulatory mechanisms of calcium ions in the uterine endometrium and conceptus are not well understood in pigs. Recently, we showed that TRPV6, a calcium ion channel protein associated with cellular entry of calcium ions, is highly expressed in the uterine endometrium during the implantation period in pigs. In the present study, we investigated spatial and temporal expression and regulation of TRPV6 and S100G, an intracellular calcium-regulatory molecule, in the uterine endometrium during the estrous cycle and pregnancy in pigs. TRPV6 expression was maintained at significantly higher levels in the uterine endometrium during pregnancy compared with levels during the estrous cycle. TRPV6 transcripts and proteins were localized mainly to luminal epithelial cells (LE) and weakly to glandular epithelial cells (GE) and chorionic membrane (CM) during pregnancy. TRPV6 expression was also detected in conceptuses on Day (D) 12 and D15. TRPV6 mRNA levels in the endometrium were increased by estrogen treatment. S100G expression showed a biphasic pattern of increases on D12 of pregnancy and from D60 to term pregnancy, and it localized primarily to LE during early pregnancy and to LE, GE, and CM from D30 to term pregnancy. These results indicate that spatial and temporal expression of TRPV6 and S100G is dynamically regulated in the uterine endometrium during pregnancy and that endometrial regulation of calcium ion concentration by TRPV6 and S100G may be critical for the establishment and maintenance of pregnancy in pigs.