EXPRESSION OF EXTRACELLULAR SUPEROXIDE-DISMUTASE BY HUMAN CELL-LINES
EXPRESSION OF EXTRACELLULAR SUPEROXIDE-DISMUTASE BY HUMAN CELL-LINES
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DOI：
10.1042/bj2660213
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发表时间：
1990-02-15
影响因子：
4.1
通讯作者：
MARKLUND, SL
中科院分区：
文献类型：
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作者：
MARKLUND, SL
细胞外超氧化物歧化酶(EC-SOD)是细胞外液中的主要超氧化物歧化酶同工酶，也存在于组织中。该酶的合成位置和特性尚不清楚。用ELISA法检测了一大批人细胞系培养中EC-SOD的存在。与细胞内铜锌超氧化物歧化酶不同的是，EC-超氧化物歧化酶只在少数几种细胞中表达。在调查的10个悬浮生长细胞系中，没有一个产生EC-SOD。在正常的二倍体锚定依赖细胞系中，所研究的25个成纤维细胞系中均有表达，在两个胶质细胞系中均有表达，但在6个内皮细胞系、2个上皮细胞系和2个羊膜来源的细胞系中未见表达。在肿瘤锚定依赖性细胞系中，29例中有13例有表达，EC-SOD由表达该酶的细胞株分泌到培养液中。不同成纤维细胞系之间EC-SOD的合成速率相差近100倍，在长期培养过程中，各成纤维细胞系之间基本保持一致。在调查的9例病例中，分泌的EC-SOD为高肝素亲和力C型。我们认为，组织EC-SOD是由一些分散的细胞类型，如成纤维细胞和胶质细胞分泌的，随后在大多数组织细胞类型的糖萼和间质基质中与硫酸乙酰肝素蛋白多糖配基可逆结合的周围扩散。
Extracellular superoxide dismutase (EC-SOD) is the major SOD isoenzyme in extracellular fluids, but occurs also in tissues. The sites and characterisitcs of the synthesis of the enzyme are unknown. The occurrence of EC-SOD in cultures of a large panel of human cell lines was assayed by means of an e.l.i.s.a. Unlike the situation for the intracellular isoenzymes CuZn-SOD, expression of EC-SOD occurs in only a few cell types. None of the ten investigated suspension-growing cell lines produced EC-SOD. Among normal diploid anchorage-dependent cell lines, expression was found in all 25 investigated fibroblast cell lines, in the two glia-cell lines, but not in six endothelial-cell lines, two epithelial-cell lines or in two ammion-derived lines. Among neoplastic anchorage-dependent cell lines expression was found in 13 out of 29, EC-SOD was secreted into the culture medium by cell lines expressing the enzyme. The rate of EC-SOD synthesis varied by nearly 100-fold among the fibroblast lines and remained essentially consistant in the individual lines during long-term culture. In the nine investigated cases, the secreted EC-SOD was the high-heparin-affinity C type. It is suggested that tissue EC-SOD is secreted by a few well-dispersed cell types, such as fibroblasts and glia cells, to diffuse subsequently around the reversibly bind to heparan sulphate proteoglycan ligands in the glycocalyx of the surfaceof most tissue cell types and in the interstitial matrix.