Characterisation of the contractile dynamics of the resting ex vivo urinary bladder of the pig
Characterisation of the contractile dynamics of the resting ex vivo urinary bladder of the pig
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DOI：
10.1111/bju.13132
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发表时间：
2015-12-01
影响因子：
4.5
通讯作者：
Chambers, John Paul
中科院分区：
文献类型：
--
作者：
Lentle, Roger G.;Reynolds, Gordon W.;Chambers, John Paul
ObjectivesTo estimate the area and movements of ongoing spontaneous localised contractions in the resting pork urinary bladder，and relate these to ambient intravesical pressure（P-ves），to further our understanding of their genesis and role in accommodating incoming urinary.Materials and MethodsWe used image analysis to quantify the areas and movements of discrete propagating patches of contraction（PPC）on the anterior，6只猪的膀胱的前外侧和后表面保持离体体积的小增量增加。然后，我们相关的P-VES和周期性变化的P-VES与参数来自spatiotemporary map.ResultsContractile运动在静息膀胱只包括PPC，覆盖约五分之一的膀胱表面，开始在不同的网站，并在持续时间约为6秒。它们以大约6 mm/s的速度传播，主要通过各种途径（有时是环形途径）以准稳定的节奏穿过膀胱的前表面和侧表面，并且不穿过三角区。这些节律的频率较低（3.15个周期/分钟），与P-ves的周期性变化（3.55个周期/分钟）大致相似。每个PPC与拉伸区域（正应变率）相关，这些事件发生在更恒定的应变背景下。P-ves突然增加后，P-ves周期的振幅和PPCs发生区的振幅增加，但P-ves周期的频率和PPCs起源的频率不变。当PPC穿过膀胱的上半部分时，周期出现峰值，这是更顺应性的。PPC的传播速度与膀胱肌细胞中动作电位的横向传播速度相似，且显著大于间质细胞中的传播速度。PPC的大小，他们的频率和他们的传播率不受动脉内剂量与河豚毒素或lidocaine.ConclusionThe起源和持续时间的PPC的影响P-VES和周期变化P-VES。因此，传播而不是静止的收缩区域可能有助于整体音调和P-ves的变化。PPCs的时空映射可能有助于我们理解张力的产生和临床实体的基础，如膀胱过度活动症，膀胱疼痛综合征和逼尿肌过度活动。
ObjectivesTo characterise the area and movements of ongoing spontaneous localised contractions in the resting porcine urinary bladder and relate these to ambient intravesical pressure (P-ves), to further our understanding of their genesis and role in accommodating incoming urine.Materials and MethodsWe used image analysis to quantify the areas and movements of discrete propagating patches of contraction (PPCs) on the anterior, anterolateral and posterior surfaces of the urinary bladders of six pigs maintained ex vivo with small incremental increases in volume. We then correlated the magnitude of P-ves and cyclic changes in P-ves with parameters derived from spatiotemporal maps.ResultsContractile movements in the resting bladder consisted only of PPCs that covered around a fifth of the surface of the bladder, commenced at various sites, and were of approximate to 6 s in duration. They propagated at around 6 mm/s, mainly across the anterior and lateral surface of the bladder by various, sometimes circular, routes in a quasi-stable rhythm, and did not traverse the trigone. The frequencies of these rhythms were low (3.15 cycles/min) and broadly similar to those of cyclic changes in P-ves (3.55 cycles/min). Each PPC was associated with a region of stretching (positive strain rate) and these events occurred in a background of more constant strain. The amplitudes of cycles in P-ves and the areas undergoing PPCs increased after a sudden increase in P-ves but the frequency of cycles of P-ves and of origin of PPCs did not change. Peaks in cycles occurred when PPCs were traversing the upper half of the bladder, which was more compliant. The velocity of propagation of PPCs was similar to that of transverse propagation of action potentials in bladder myocytes and significantly greater than that reported in interstitial cells. The size of PPCs, their frequency and their rate of propagation were not affected by intra-arterial dosage with tetrodotoxin or lidocaine.ConclusionThe origin and duration of PPCs influence both P-ves and cyclic variation in P-ves. Hence, propagating rather than stationary areas of contraction may contribute to overall tone and to variation in P-ves. Spatiotemporal mapping of PPCs may contribute to our understanding of the generation of tone and the basis of clinical entities such as overactive bladder, painful bladder syndrome and detrusor overactivity.