An improved HPLC method for simultaneous determination of phenolic compounds, purine alkaloids and theanine in Camellia species
An improved HPLC method for simultaneous determination of phenolic compounds, purine alkaloids and theanine in Camellia species
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DOI：
10.1016/j.jfca.2008.05.002
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发表时间：
2008-11-01
影响因子：
4.3
通讯作者：
Ye, Chuangxing
中科院分区：
文献类型：
--
作者：
Peng, Li;Song, Xiaohong;Ye, Chuangxing
建立了一种简便、快速的高效液相色谱（HPLC）分析茶花中酚类化合物、嘌呤类生物碱和茶氨酸的方法。采用酰胺- c16色谱柱，梯度洗脱系统可在45 min内快速分离儿茶素、没食子酸、咖啡因、可可碱、茶碱、茶碱和茶氨酸。14种化合物在研究范围内均具有良好的线性关系，相关系数在0.9994 ~ 0.9999之间。14种化合物的检出限和定量限分别为0.0001 ~ 0.072 ng/ μ L和0.0004 ~ 0.24 ng/ μ L。用该方法对4种茶花进行了分析。在传统栽培的茶树、山茶和assamica中，主要的嘌呤生物碱为咖啡因，主要的酚类化合物为(-)-表没食子儿茶素没食子酸酯（EGCG）。山茶和苦茶的主要嘌呤生物碱为可可碱和茶碱；其含量分别为4.001 +/- 0.1184%和2.116 +/- 0.0270%。苦参和苦参的主要酚类化合物分别为(-)-没食子儿茶素没食子酸酯（GCG）和EGCG。四种茶花样品中的茶氨酸含量范围为0.136 +/- 0.0026%至1.485 +/- 0.0491%干重，英国皇家版权(c) 2008由爱思唯尔公司出版。版权所有。
A simple and fast HPLC analysis method for phenolic compounds, purine alkaloids and theanine in various Camellia species was developed. Using an amide-C16 column, catechins, gallic acid, caffeine, theobromine, theacrine, theophyline and theanine could be rapidly separated within 45 min with a gradient elution system. Excellent linearity was observed for all 14 compounds in the range studied, with correlation coefficients between 0.9994 and 0.9999. Limit of detection and limit of quantification of the 14 compounds varied from 0.0001 to 0.072 ng/mu L and 0.0004 to 0.24 ng/mu L, respectively. Four kinds of Camellia species were analyzed using this method. In traditional cultivated tea trees, Camellia sinensis and Camellia assamica, the main purine alkaloid was caffeine and main phenolic compound was (-)-epigallocatechin gallate (EGCG). The main purine alkaloids in Camellia ptilophylla and C. assamica var. kucha were theobromine and theacrine; their contents were 4.001 +/- 0.1184% dry weight and 2.116 +/- 0.0270% dry weight, respectively. The main phenolic compounds in C. ptilophylla and C. assamica var. kucha were (-)-gallocatechin gallate (GCG) and EGCG respectively. The content of theanine in the four Camellia species samples ranged from 0.136 +/- 0.0026% to 1.485 +/- 0.0491% dry weight, Crown Copyright (c) 2008 Published by Elsevier Inc. All rights reserved.