Coordinate regulation of transcription and splicing by steroid receptor coregulators
Coordinate regulation of transcription and splicing by steroid receptor coregulators
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DOI：
10.1126/science.1073734
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发表时间：
2002-10-11
期刊：
影响因子：
56.9
通讯作者：
O'Malley, BW
中科院分区：
文献类型：
--
作者：
Auboeuf, D;Hönig, A;O'Malley, BW
最近的观察表明，启动子身份会影响替代性 RNA 加工决策，这引起了人们对 RNA 聚合酶 II 转录和前体信使 RNA (pre-mRNA) 加工之间调节相互作用的兴趣。我们研究了类固醇受体介导的转录对RNA加工的影响，报告基因受到类固醇敏感启动子驱动的选择性剪接。类固醇激素以类固醇受体依赖性和受体选择性的方式影响由类固醇敏感启动子合成的前mRNA的加工，但不影响由类固醇无反应启动子合成的前mRNA的加工。几种核受体共调节子表现出不同的剪接效应，表明类固醇激素受体可能通过招募参与这两个过程的共调节子来同时控制基因转录活性和产物mRNA的外显子含量。
Recent observations indicating that promoter identity influences alternative RNA-processing decisions have created interest in the regulatory interactions between RNA polymerase II transcription and precursor messenger RNA (pre-mRNA) processing. We examined the impact of steroid receptor mediated transcription on RNA processing with reporter genes subject to alternative splicing driven by steroid- sensitive promoters. Steroid hormones affected the processing of pre-mRNA synthesized from steroid-sensitive promoters, but not from steroid-unresponsive promoters, in a steroid receptor dependent and receptor-selective manner. Several nuclear receptor coregulators showed differential splicing effects, suggesting that steroid hormone receptors may simultaneously control gene transcription activity and exon content of the product mRNA by recruiting coregulators involved in both processes.