Long non-coding RNA MALAT1/microRNA 125a axis presents excellent value in discriminating sepsis patients and exhibits positive association with general disease severity, organ injury, inflammation level, and mortality in sepsis patients
Long non-coding RNA MALAT1/microRNA 125a axis presents excellent value in discriminating sepsis patients and exhibits positive association with general disease severity, organ injury, inflammation level, and mortality in sepsis patients
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DOI：
10.1002/jcla.23222
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发表时间：
2020-04-20
影响因子：
2.7
通讯作者：
Yu, Li
中科院分区：
文献类型：
--
作者：
Liu, Wei;Geng, Feng;Yu, Li
目的探讨长链非编码RNA转移相关肺腺癌转录本1 (lnc-MALAT1)/microRNA (miR)-125a轴在脓毒症疾病管理和预后监测中的潜在价值。方法选取196例败血症患者和196例健康对照。脓毒症患者入院后24小时内采集血样，健康对照者入组时采集血样。采用逆转录定量聚合酶链反应检测lnc-MALAT1和miR-125a在所有参与者中的相对表达，采用酶联免疫吸附法检测脓毒症患者血浆中的炎症细胞因子。结果脓毒症患者的Lnc-MALAT1/miR-125a轴与健康对照相比增高（P < 0.001），曲线下面积（AUC）为0.931 (95% CI: 0.908 ~ 0.954)，对脓毒症患者与健康对照的鉴别具有很好的价值。在脓毒症患者中，lnc-MALAT1与miR-125a呈负相关，lnc-MALAT1/miR-125a轴与急性病理和慢性健康评估II （APACHE II）评分、顺序器官衰竭评估（SOFA）评分、血清肌酐、c反应蛋白、肿瘤坏死因子α、白细胞介素(IL)-1 β、IL-6、IL-8呈正相关，与白蛋白呈负相关。此外，lnc-MALAT1/miR-125a轴在一定程度上预测28天死亡风险增加有价值（AUC: 0.678, 95% CI: 0.603-0.754）。结论Lnc-MALAT1/miR-125a轴在脓毒症患者与健康对照的鉴别中具有很好的价值，并且与脓毒症患者的一般疾病严重程度、器官损伤、炎症水平和死亡率呈正相关。
Objective The present study aimed to investigate the potential value of long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (lnc-MALAT1)/microRNA (miR)-125a axis in disease management and prognosis surveillance of sepsis.Methods Totally, 196 sepsis patients and 196 healthy controls were enrolled. Blood samples were collected within 24 hours after admission in sepsis patients and were collected at enrollment in healthy controls. The relative expression of lnc-MALAT1 and miR-125a in all participants was detected by reverse transcription quantitative polymerase chain reaction, and the inflammatory cytokines in plasma of sepsis patients were measured by enzyme-linked immunosorbent assay.Results Lnc-MALAT1/miR-125a axis was increased in sepsis patients compared with healthy controls (P < .001) and was of excellent value in distinguishing septic patients from healthy controls with the area under the curve (AUC) of 0.931 (95% CI: 0.908-0.954). In sepsis patients, lnc-MALAT1 was negatively associated with miR-125a, and lnc-MALAT1/miR-125a axis was positively correlated with acute pathologic and chronic health evaluation II (APACHE II) score, Sequential Organ Failure Assessment (SOFA) score, serum creatinine, C-reactive protein, tumor necrosis factor-alpha, interleukin (IL)-1 beta, IL-6, and IL-8, while negatively associated with albumin. Furthermore, lnc-MALAT1/miR-125a axis was of value in predicting increased 28-day mortality risk to some extent (AUC: 0.678, 95% CI: 0.603-0.754).Conclusion Lnc-MALAT1/miR-125a axis presents excellent value in differentiating sepsis patients from healthy controls and also exhibits positive association with general disease severity, organ injury, inflammation level, and mortality in sepsis patients.