Use of a commercial ELISA for the detection of measles-specific immunoglobulin G (IgG) in dried blood spots collected from children living in low-resource settings
Use of a commercial ELISA for the detection of measles-specific immunoglobulin G (IgG) in dried blood spots collected from children living in low-resource settings
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DOI：
10.1002/jmv.24136
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发表时间：
2015-09-01
影响因子：
12.7
通讯作者：
Mokdad, Ali H.
中科院分区：
文献类型：
--
作者：
Colson, K. Ellicott;Potter, Alan;Mokdad, Ali H.
对人群对疫苗可预防疾病的免疫力进行血清流行病学监测对预防未来暴发至关重要。干燥血斑(DBS)是在滤纸上干燥的毛细血管血滴，是一种负担得起的、微创的替代静脉穿刺法，用于现场采集血液。然而，几乎没有经过验证的方法来分析DBS是否存在保护性抗体。本研究验证了一种使用商业ELISA检测毛细血管DBS中麻疹特异性免疫球蛋白G(Ig G)的新技术。通过比较50名儿童匹配的血清和DBS样本，测试了一种新的DBS分析方法的预测性能。对该方法的准确性、精密度和可靠性进行了评价，并确定了对阳性和阴性样品进行分类的最佳切割点。然后，该方法被应用于在墨西哥和尼加拉瓜对儿童进行大规模调查期间收集的1588个DBS。血清样本中麻疹特异性抗体62%为阴性，10%为可疑，28%为阳性。与配对血清比较，DBS结果100%敏感，968%特异，50例中有46例符合(92%)。试剂盒提供的对照的批间和批内变异系数大于预期(分别为24.8%和8.4%)。然而，在预测性模拟中，平均错误分类只有3.9%。调查人员和参与者均可接受检查程序。分析在低资源环境下收集的数据库是衡量人群麻疹免疫力的一种可行和准确的手段，应用于产生健康状况和卫生系统绩效的客观衡量标准。J.Med.维罗尔。2015年，87：1491-1499。(C)2015年威利期刊公司。
Seroepidemiological monitoring of population immunity to vaccine-preventable diseases is critical to prevent future outbreaks. Dried blood spots (DBS), drops of capillary blood dried on filter paper, are an affordable, minimally invasive alternative to venipuncture for collecting blood in field settings. However, few proven methods exist to analyze DBS for the presence of protective antibodies. This study validates a novel technique for measuring measles-specific immunoglobulin G (IgG) in capillary DBS using a commercial ELISA. The predictive performance of a new method for analyzing DBS was tested by comparing matched serum and DBS samples from 50 children. The accuracy, precision, and reliability of the procedure were evaluated, and the optimal cut points to classify positive and negative samples were determined. The method was then applied to 1,588 DBS collected during a large survey of children in Mexico and Nicaragua. Measles-specific IgG in serum samples were 62% negative, 10% equivocal, and 28% positive. In comparisons with matched serum, DBS results were 100% sensitive and 968% specific, and agreed in 46 of 50 (92%) cases. The inter-assay and intra-assay coefficients of variation from kit-provided controls were greater than desired (24.8% and 8.4%, respectively). However, in predictive simulations the average misclassification was only 3.9%. Procedures were found to be acceptable to surveyors and participants. Analyzing DBS collected in low-resources settings is a feasible and accurate means of measuring population immunity to measles and should be used to generate objective measures of health status and health system performance. J. Med. Virol. 87:1491-1499, 2015. (c) 2015 Wiley Periodicals, Inc.