Cloning and RNAi-mediated three lethal genes that can be potentially used for Chilo suppressalis (Lepidoptera: Crambidae) management
Cloning and RNAi-mediated three lethal genes that can be potentially used for Chilo suppressalis (Lepidoptera: Crambidae) management
复制标题
克隆和 RNAi 介导的三个致死基因可潜在用于二化螟（鳞翅目：Crambidae）管理
DOI：
10.1016/j.pestbp.2021.104828
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发表时间：
2021-04-08
影响因子：
4.7
通讯作者：
Ma, Weihua
中科院分区：
文献类型：
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作者：
Jin, Huihui;Abouzaid, Mostafa;Ma, Weihua
近年来，RNA干扰（RNAi）作为一种可行的害虫控制策略受到关注。在这里，RNAi检测进行筛选基因的潜在功能，二化螟，水稻的严重害虫，并确定他们的潜力，开发一个高度针对性的分子控制方法。从C. suppressalis，并评估它们的时空基因表达。所有这三个基因的表达在蛹和成虫阶段比幼虫阶段更高，并且在幼虫头部比其他组织更高。新孵化的幼虫表现出高死亡率和抑制生长时，喂养细菌产生的双链RNA（dsRNA）对应的三个靶基因。本研究为深入了解ND、GPDH和MSL3在C.这些基因可能是二化螟幼虫发育的候选基因靶点。二化螟害虫治理
RNA interference (RNAi) has gained attention in recent years as a viable pest control strategy. Here, RNAi assays were performed to screen the potential functionality of genes in Chilo suppressalis, a serious pest of rice, and to determine their potential for developing a highly targeted molecular control approach. Potential homologs of NADH dehydrogenase (ND), glycerol 3-phosphate dehydrogenase (GPDH) and male specific lethal 3 (MSL3) were cloned from C. suppressalis, and their spatiotemporal gene expression evaluated. The expression of all three genes was higher in the pupal and adult stages than the larval stages and largely higher in the larval head compared to other tissues. Newly hatched larvae exhibited high mortalities and suppressed growth when fed bacteria producing double-stranded RNAs (dsRNAs) corresponding to the three target genes. This study provides insights into the function of ND, GPDH and MSL3 during C. suppressalis larval development and suggests that all may be candidate gene targets for C. suppressalis pest management.