Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells
Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells
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DOI：
10.1126/science.277.5323.221
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发表时间：
1997-07-11
期刊：
影响因子：
56.9
通讯作者：
Wirth, T
中科院分区：
文献类型：
--
作者：
Zwilling, S;Dieckmann, A;Wirth, T
BOB.1/OBF.1是一种转录共激活因子，在B细胞中组成型表达，并与Oct 1和Oct 2转录因子相互作用。在用佛波醇酯和离子霉素活化Jurkat T细胞和原代鼠胸腺细胞后，BOB.1/OBF. 1表达和反式激活功能被诱导。BOB.1/OBF.1在体内和体外都在Ser（184）处磷酸化，并且这种修饰是诱导活化所需的。Ser（184）突变也降低了B细胞中的反式激活功能，表明T细胞中可诱导的激活磷酸化组成性地存在于B细胞中。因此，BOB.1/OBF.1是一种转录辅激活因子，受翻译后修饰的严格调控，介导细胞类型特异性基因表达。
BOB.1/OBF.1 is a transcriptional coactivator that is constitutively expressed in B cells and interacts with the Oct1 and Oct2 transcription factors. Upon activation of Jurkat T cells and primary murine thymocytes with phorbol esters and ionomycin, BOB.1/OBF.1 expression and transactivation function were induced. BOB.1/OBF.1 was phosphorylated at Ser(184) both in vivo and in vitro, and this modification was required for inducible activation. Mutation of Ser(184) also diminished transactivation function in B cells, suggesting that the activating phosphorylation that is inducible in T cells is constitutively present in B cells. Thus, BOB.1/OBF.1 is a transcriptional coactivator that is critically regulated by posttranslational modifications to mediate cell type-specific gene expression.