SOMATIC HYPERMUTAGENESIS IN IMMUNOGLOBULIN GENES .2. INFLUENCE OF NEIGHBORING BASE SEQUENCES ON MUTAGENESIS
SOMATIC HYPERMUTAGENESIS IN IMMUNOGLOBULIN GENES .2. INFLUENCE OF NEIGHBORING BASE SEQUENCES ON MUTAGENESIS
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DOI：
10.1016/0167-4781(92)90134-l
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发表时间：
1992-11-15
期刊：
影响因子：
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通讯作者：
KOLCHANOV, NA
中科院分区：
文献类型：
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作者：
ROGOZIN, IB;KOLCHANOV, NA
描述了一种分析突变热点的新方法。它是基于热点位点序列的分类。利用该方法，揭示了V基因热点位点的一致RGYW和TAA。采用统计权重法对14个V基因的323个体细胞替换进行了体细胞突变与这些共识的相关性研究。假设没有任何相关性，在样本中观察到此类数据的概率将非常低（0.0003）。这些结果支持体细胞突变的出现受到邻近碱基序列的显著影响的观点。对V基因侧翼296个体细胞突变的分析也支持了这一观点。据推测，这种影响是体细胞超诱变的一个重要特征。
A new approach for the analysis of hotspots of mutations is described. It is based on the classification of hotspot site sequences. Using this approach, the consensuses RGYW and TAA of hotspot sites were revealed in the V gene. Correlation between somatic mutations and these consensuses is investigated by the statistical weight method in 323 somatic substitutions in 14 V genes. Assuming the absence of any correlation, the probability of observing such data in the sample would be very low (0.0003). These results support the idea that emergence of somatic mutation is significantly influenced by neighbouring base sequences. This idea was also supported by the analysis of 296 somatic mutations in flanking sequences of V genes. It is supposed that this influence is an important feature of somatic hypermutagenesis.