The fibrillar structure of cement lines on resorbed root surfaces of human teeth.
The fibrillar structure of cement lines on resorbed root surfaces of human teeth.
复制标题
人类牙齿吸收根表面上的水泥线的纤维结构。
DOI：
10.1034/j.1600-0765.2000.035004208.x
复制
发表时间：
2000
影响因子：
3.5
通讯作者：
M. N. Islam
中科院分区：
文献类型：
--
作者：
T. Yamamoto;T. Domon;S. Takahashi;R. Suzuki;M. N. Islam
应用光镜和扫描电镜结合氢氧化钠溶液浸泡法，观察了人牙修复性牙骨质与吸收牙本质或牙骨质之间的胶结线。采用NaOH浸渍法去除纤维间物质，直接观察水泥线的纤维结构。光镜观察显示，骨水泥线富含蛋白多糖和粘多糖，但缺乏胶原纤维。在用透明质酸酶处理后，人工破坏骨水泥线，透明质酸酶可吸收一些粘多糖，但不吸收胶原纤维。扫描电子显微镜显示，纤维混合只发生在修复性牙骨质和吸收组织之间的某些地方。这些发现表明，骨水泥线中的蛋白多糖介导了新旧矿化组织之间的附着。
The cement lines between reparative cementum and resorbed dentin or cementum in human teeth were observed by light microscopy and scanning electron microscopy combined with NaOH maceration. The NaOH maceration was used to remove interfibrillar substances and to observe the fibrillar architecture of the cement lines directly. Light microscopy showed that the cement lines were rich in proteoglycans with mucopolysaccharides, but deficient in collagen fibrils. The cement lines were artificially broken after treatment with hyaluronidase, which digests some of the mucopolysaccharides, but digests no collagen fibrils. Scanning electron microscopy showed that fibril intermingling occurred only in some places between reparative cementum and resorbed tissue. These findings suggested that the proteoglycans in cement lines mediate the attachment between new and old mineralized tissue.