Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: a Dethiobiotin producing Bacterium
Characterization of Biotin Biosynthetic Enzymes of Bacillus sphaericus: a Dethiobiotin producing Bacterium
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球形芽孢杆菌生物素生物合成酶的表征：一种产生脱硫生物素的细菌
DOI：
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发表时间：
1981
期刊：
影响因子：
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通讯作者：
Hideaki Yamada
中科院分区：
文献类型：
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作者：
Y. Izumi;Y. Kano;K. Inagaki;Naoki Kawase;Y. Tani;Hideaki Yamada
检查了多种细菌和酵母的生物素生物合成酶的活性，包括庚二酰辅酶A合成酶、7-酮-8-氨基壬酸（KAPA）合成酶、7,8-二氨基壬酸（DAPA）氨基转移酶和脱硫生物素（DTB）合成酶。在测试的菌株中，只有球形芽孢杆菌（DTB 生产者）对所有四种酶都表现出显着的活性。该细菌还在完整的细胞系统中表现出从 DTB 合成生物素的高活性。利用无细胞提取物和完整细胞，检测了DAPA转氨酶、DTB合成酶和生物素合成反应的一些性质。根据这些酶活性结果，讨论了球形芽孢杆菌的DTB生产力。
A variety of bacteria and yeasts were examined for activities of biotin biosynthetic enzymes, including pimelyl-CoA synthetase, 7-keto-8-aminopelargonic acid (KAPA) synthetase, 7,8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin (DTB) synthetase. Among the strains tested, only Bacillus sphaericus, a DTB producer, showed significant activities for all four enzymes. The bacterium also exhibited high activity of biotin synthesis from DTB in an intact cell system. Using cell-free extract and intact cells, some properties of DAPA aminotransferase, DTB synthetase and biotin synthesizing reaction were examined.Based on these results of enzyme activities DTB productivity of B. sphaericus was discussed.