A fluorescent sensor for the real-time measurement of extracellular oxytocin dynamics in the brain
A fluorescent sensor for the real-time measurement of extracellular oxytocin dynamics in the brain
复制标题
用于实时测量大脑细胞外催产素动态的荧光传感器
DOI：
10.1101/2021.07.30.454450
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Nishiyama Masaaki
中科院分区：
文献类型：
--
作者：
Ino Daisuke;Hibino Hiroshi;Nishiyama Masaaki
催产素（OT）是一种下丘脑神经肽，在大脑中起神经调节剂的作用，协调各种动物行为。然而，大脑OT动力学和复杂动物行为之间的关系在很大程度上仍然难以捉摸，部分原因是缺乏一种合适的技术来实时记录其在体内的活动。在这里，我们描述了MTRIAOT，一种基于g蛋白偶联受体的绿色荧光OT传感器，具有大的动态范围，合适的亲和力，对OT同源物的配体特异性，对下游信号传导的影响最小和长期荧光稳定性。通过结合病毒基因传递和纤维光度法介导的荧光测量，我们证明了mtriaot在活体小鼠脑OT动态实时检测中的实用性。mtriaot介导的测量表明，OT动力学的可变性取决于动物的行为背景和身体状况。mtriaot将有可能分析各种生理和病理过程中的OT动力学。
Oxytocin (OT), a hypothalamic neuropeptide that acts as a neuromodulator in the brain, orchestrates a variety of animal behaviors. However, the relationship between brain OT dynamics and complex animal behaviors remains largely elusive, partly because of the lack of a suitable technique for its real-time recording in vivo. Here, we describe MTRIAOT, a G-protein-coupled receptor-based green fluorescent OT sensor that has a large dynamic range, suitable affinity, ligand specificity for OT orthologs, minimal effects on downstream signaling and long-term fluorescence stability. By combining viral gene delivery and fiber photometry-mediated fluorescence measurements, we demonstrate the utility of MTRIAOTfor real-time detection of brain OT dynamics in living mice. MTRIAOT-mediated measurements indicate variability of OT dynamics depending on the behavioral context and physical condition of an animal. MTRIAOTwill likely enable the analysis of OT dynamics in a variety of physiological and pathological processes.